Buletin Veteriner Udayana
Bul. Vet. Udayana. October 2024 Vol. 16 No. 5

HISTOLOGY AND PROFILE OF AGRANULOCYTE OF BALI CATTLE WITH CONVENTIONAL REARED SYSTEM

Putri Utami (Mahasiswa Sarjana Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234)
Ni Ketut Suwiti (Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia)
I Nyoman Suartha (Laboratorium Ilmu Penyakit Dalam Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. Raya Sesetan Gg. Markisa No. 6, Denpasar Selatan, Bali, Indonesia)
Ni Luh Eka Setiasih (Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia)
Ni Nyoman Werdi Susari (Laboratorium Anatomi dan Embriologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali, Indonesia)
Anak Agung Sagung Kendran (Laboratorium Patologi Klinik Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234)



Article Info

Publish Date
09 Sep 2024

Abstract

Bali cattle are one of the livestock that are currently widely bred by farmers in Bali. Generally, Bali cattle are raised conventionally or traditionally and pay less attention to the health of the cattle. This situation can affect the immune system of Bali cattle, especially white blood cells that play a role in the immune system such as lymphocytes and monocytes. This study aims to determine the histomorphometry of white blood cells agranulocytes lymphocytes and monocytes in Bali cattle with conventional reared system. Blood test preparations were taken from 30 cows in Bulian Village, Kubutambahan Subdistrict, Buleleng Regency, Bali. Fixation and staining using MDT IndoReagen®. Measurements were made using the EP View application connected to a microscope with 1000x magnification. Calculation of the number of lymphocytes and monocytes by counting up to 100 white blood cells. The results showed that the histomorphology of lymphocytes has an irregular shape, a thin blue-purplish cytoplasm, with a nucleus that almost fills the cytoplasm. Lymphocytes have an area of 17.56 ± 2.37 µm2, perimeter of 14.7 ± 1.14 µm, and diameter of 4.71 ± 0.33 µm. The histomorphology of monocytes has an irregular shape, the cytoplasm is not granular and the nucleus is found hollow. Monocytes have an area of 22.59±5.09 µm2, perimeter of 16.69±2.37 µm, and diameter of 5.19±0.66 µm. Further research is needed on the histomorphometry of granulocytes: neutrophils, basophils, and eosinophils of Bali cattle.

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Journal Info

Abbrev

buletinvet

Publisher

Subject

Immunology & microbiology Public Health Veterinary

Description

FOCUS The journal focused on Veterinary Medicine, Animal Sciences, and Health Studies with various developments. SCOPE Zoonoses, Public Health, One Health, Epidemiology, Reproduction, Virology, Bacteriology, Parasitology, Mycology, Internal Medicine, Surgery, Orthopedics, Vaccines, Genetics, ...