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All Journal Buletin Veteriner Udayana
Ni Nyoman Werdi Susari
Laboratorium Anatomi dan Embriologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali, Indonesia
Immunology & microbiology Public Health Veterinary

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EVALUATION OF PRESERVED ORGANS OF PLASTINATION WITH THE DEHYDRATION PHASE AT ROOM TEMPERATURE Ni Nyoman Werdi Susari; I Nengah Wandia; I Ketut Suatha; Luh Gde Sri Surya Heryani
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p26

Abstract

Organ preservation is an effort to maintain the integrity of organs for a long time by preventing further damage and decay processes. Plastination is an organ preservation process by inserting polymer materials to maintain the shape and composition of the organ. This research aims to evaluate the product of a plastination technique carried out using generic chemicals in the community and equipment. The organs used in this plastination process are heart, brain and lungs from the bali cattle. The research materials and tools needed are distilled water, formalin, acetone, liquid silicone rubber, silicon catalyst, 10 ml syringe, plastic bag/tub with lid, vacuum chamber, plastic bucket/tub without lid, and hardening room/box. The vacuum chamber is made from a modified drum so that it can accommodate cadaver plastination (large size). The plastination technique is carried out in four main stages, one of which is the dehydration phase with acetone which is carried out at room temperature. The plastinated organs that are the product of this technique will be evaluated for their flexibility, color, and odor. The results of this research produce plastinated organs that have a stiff texture, pale color, and a non-pungent odor. From the research results obtained, it can be suggested that it needs to be stored in a vacuum for longer (for 1 week) so that the texture can resemble its original shape.  
HISTOMORPHOMETRI OF WHITE BLOOD CELLS OF BALI DUCKS USING HISTOCHEMICAL METHODS I Gusti Ngurah Jagad Anom Ksatriya; Ni Ketut Suwiti; Anak Agung Sagung Kendran; Ni Luh Eka Setiasih; I Ketut Suatha; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p25

Abstract

Bali ducks are one of the local poultry breeds whose meat and eggs are usually used. Bali ducks can experience immune disorders, especially their susceptibility to disease. White blood cells can be used as an indicator of the infection in the body, so white blood cell examination is necessary to evaluate livestock health. This study aims to determine the histomorphometric structure and differences in white blood cells in male and female Bali ducks. This research used blood samples from 8 male Balinese ducks and 8 female Balinese ducks aged two to three months from farms in Mengwi District, Badung Regency. Staining of blood smear was carried out using eosin and methylene blue staining (MDT IndoReagen®). Examination and measurement of blood cell preparations were carried out using an Olympus CX33 microscope and EPView application. Data analysis was carried out using independent samples T-test with the help of SPSS software. The results of histomorphometric examination showed that the heterophyll diameter of male Bali ducks was 5.38±0.62 µm, and the female Bali ducks was 5.23±0.60 µm. The eosinophil diameter of male Bali ducks was 5.49±0.62 µm, and the female Bali ducks was 4.99±0.54 µm. The basophil diameter of male Bali duck was 3.82±0.35 µm, and the female Bali duck was 4.33±0.52 µm. The monocyte diameter of male Bali duck was 5.13±0.72 µm, and the female Bali duck was 4.99±0.37 µm. The lymphocytes diameter of male Bali duck was 4.18±0.74 µm, and the female Bali duck was 4.52±0.58 µm. Based on the research results, it can be concluded that there is no histomorphometric difference between the white blood cells of male and female Bali ducks (P>0.05). Further research is needed regarding the histomorphometric comparison of white blood cells in Bali ducks at different ages to obtain more complete data.
HISTOPATOLOGICAL OF WHITE RATS' KIDNEY CAUSED BYAPPLICATION OF MIMOSIN FROM SIMPLISIA OF THE LEUCAENA LEAF Gusti Agung Ayu Alecia Putri Anugrahaeni; I Ketut Berata; Ida Bagus Made Oka; Ni Nyoman Werdi Susari; Ni Luh Eka Setiasih; Luh Made Sudimartini
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i03.p03

Abstract

Mimosine is a toxic substance contained in Leucaena leucocephala leaves. The toxic effects of mimosine can disrupt several tissues and organs, especially the kidneys. This study aims to determine the histopathology of white rat kidney exposed to mimosine with different doses. Male white rats with Wistar strain, 2 months old and 300-350 g body weight were being used in this study. The 20  rats used were divided into four treatment groups, namely P0 (negative control), P1 (positive control, given standard mimosine 15 mg/head/day), P2 given leucena leaf simplisia at a dose of 50 mg/head/day orally, P3 given leucaena leaf simplisia at a dose of 150 mg/head/day. On the 16th day of necropsy, the skin organs were taken and placed in 10% NBF. After the kidney organs were fixed, histopathology preparations were made using HE staining. Histopathological examination was performed including three lesion variables, namely congestion, bleeding, and inflammation. The severity of the lesions were   scored as 0, 1, 2 and 3 respectively for normal, mild, moderate and severe lesions. Data were analyzed using non-parametric Kruskal-Wallis and Mann- Whitney tests. The results of the research showed that the mimosine substance in leucaena leaf simplicia caused some histopathological changes in the kidneys of white rats like congestion, bleeding and inflammation and there was a difference in dose effect for each giving of mimosine from leucaena leaf simplicia, between the dose of 50 mg/head/day and the 150 mg/head/day. Administration of mimosine from leucaena leaf simplicia can cause histopathological changes in the kidneys of white rats and there is an effect of the difference in the dose of mimosine from leucaena leaf simplicia between a dose of 50 mg/head/day and a dose of 150 mg/day. tail/day on changes in kidney pathology of white rats, especially in the form of bleeding and inflammation. With this research, it hoped that in the future further research can be carried out on leucaena leaf simplicia with a longer administration period so that the results obtained are more accurate.
HISTOMORPHOMETRY OF THE SUPERFICIAL PECTORALIS MUSCULAR AND CRANIAL TIBIALIS MUSCULAR OF BALI DUCKS IN THE GROWTH PHASE I Kadek Ari Satria Prayoga; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p11

Abstract

Superficial pectoralis muscle is a chest muscle that is located on the surface and functions in wing movement. Tibialis cranialis muscle is the top muscle in the calf muscle structure, which functions to support the bird's body. This study aims to determine the histomorphometry of the superficial pectoralis muscle and cranial tibial muscle of male and female Bali ducks in the growing phase. This research used 20 Balinese ducks aged 12 weeks. Direct anatomical examination and histological structure with a binocular light microscope. Histomorphometry was measured using the Olympus Cellsens Standard application. Anatomy and histology results were analyzed using descriptive qualitative analysis, and histomorphometry using the ANOVA test with mean estimation. Histological structure of the superficial pectoralis muscle and cranial tibial muscle consists of muscle fibers, fasciculus, endomysium, perimysium and epimysium connective tissue. Histomorphometry of fascicle size, perimysium connective tissue thickness, and superficial pectoralis muscle endomysium were significantly different (P<0.05). Histomorphometry of the size of the fasciculus, perimysium connective tissue and endomysium of the cranial tibial muscle was not significantly different (P>0.05) in different genders. It can be concluded that the superficial pectoralis muscle and cranial tibial muscle of males and females in the growing phase are the same in terms of anatomical structure, but the size of the histological structure is different. Histomorphometry of the superficial pectoralis muscle of male and female Bali ducks is significantly different (P<0.05), but not for the tibialis cranialis muscle. Further research is needed regarding the muscles of Bali ducks at other ages.
STRUCTURE AND MORPHOMETRY OF BALI DUCK PANCREAS IN THE GROWER PHASE I Gde Andhika Putra Pratama; Ni Luh Eka Setiasih; Sri Kayati Widyastuti; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p12

Abstract

The pancreas is part of the digestive system which acts as a digestive aid organ. The pancreas has two functions, namely exocrine and endocrine. This study aims to determine the structure and morphometry of the pancreas of Bali ducks (Anas Sp) of different sexes in the growing phase. This research used 18 Balinese ducks which were divided into 2 groups, namely male and female with 9 each (3 months old). The method for examining anatomical structures is carried out by direct observation and histological structures using a binocular light microscope. Morphometry was measured using calipers for length and width, scales for pancreatic weight, and measuring cups for volume. Histomorphometry was measured using the Olympus cellSens Standard application. The results of the anatomical and histological structure data were analyzed using qualitative descriptive analysis, while the morphometric data used the Independent Sample T-Test. The anatomical structure of the Bali duck pancreas is flat like a lobed tongue and pink in colour, consisting of a capsule, islets of Langerhans, acini, intercalary ducts, intralobular ducts, interlobular ducts, intralobular septa, interlobular septa, veins and arteries. The results of morphometric measurements of Bali duck pancreas showed significant differences (P<0.05) in the weight and volume of the pancreas. Histomorphometric measurements of the area of the islets of Langerhans, the area of the acini, the thickness of the intralobular septa, and the thickness of the interlobular septa showed that they were not significantly different (P>0.05) in both sexes. The anatomical structure and histology of the pancreas of male and female Bali ducks are the same, but there are differences in morphometry
HISTOMORPHOMETRY OF THE DEXTER VENTRICLE OF LOCAL BALI DUCKS AT GROWTH PHASE Delima Marsinta Ida Pasaribu; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; I Ketut Berata; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p20

Abstract

Heart is the main organ in blood circulation which plays a role in pumping blood to all body cells and returning to the heart. Avian heart has four chambers consisting of two atria (dexter and sinister) and two ventricles (dexter and sinister), each chamber has three layers of walls consisting of epicardium, myocardium and endocardium. This study aimed to obtain information about the histological structure and histomorphometry of ventriculus dexter of the bali duck’s heart at grower phase. The study sample used 18 bali ducks, which were divided into two gender groups and each consisted of 9 ducks (3 months old). Ventriculus dexter sample was the processed into a histology preparation by Haematoxylin-Eosin (HE) staining. Anatomy and histology results were analyzed using descriptive qualitative analysis, while histomorphometry used the anova test with mean estimation using the SPSS 26 program. The mean thickness of the epicardium, myocardium, and endocardium in males respectively were 43,11 ± 12,05µm, 1.145,19 ± 133,99µm, 23,41 ± 6,91µm. While in females respectively were 34,98 ± 17,03µm, 1.367,41 ± 412,43µm, 11,86 ± 5,06µm. Histomorphometric data that has been analyzed shows that the results of measuring the thickness of the endocardium are significantly different (P<0,05), while measuring the thickness of the epicardium and myocardium was not significantly different (P>0,05).. It can be concluded that the anatomical structure of the heart and the histological structure of the ventriculus dexter of bali ducks didn’t differ between treatment males and females, but there were differences in the histomorphometry of the endocardial layer. Research on the heart of balinese ducks is still rarely carried out, so further research is needed on the structure and histomorphometry of the epicardium layer, myocardium layer, and endocardium layer of the dexter ventricle or other heart chambers in bali ducks at different phases.
HISTOLOGY AND PROFILE OF AGRANULOCYTE OF BALI CATTLE WITH CONVENTIONAL REARED SYSTEM Putri Utami; Ni Ketut Suwiti; I Nyoman Suartha; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2024 Vol. 16 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i05.p04

Abstract

Bali cattle are one of the livestock that are currently widely bred by farmers in Bali. Generally, Bali cattle are raised conventionally or traditionally and pay less attention to the health of the cattle. This situation can affect the immune system of Bali cattle, especially white blood cells that play a role in the immune system such as lymphocytes and monocytes. This study aims to determine the histomorphometry of white blood cells agranulocytes lymphocytes and monocytes in Bali cattle with conventional reared system. Blood test preparations were taken from 30 cows in Bulian Village, Kubutambahan Subdistrict, Buleleng Regency, Bali. Fixation and staining using MDT IndoReagen®. Measurements were made using the EP View application connected to a microscope with 1000x magnification. Calculation of the number of lymphocytes and monocytes by counting up to 100 white blood cells. The results showed that the histomorphology of lymphocytes has an irregular shape, a thin blue-purplish cytoplasm, with a nucleus that almost fills the cytoplasm. Lymphocytes have an area of 17.56 ± 2.37 µm2, perimeter of 14.7 ± 1.14 µm, and diameter of 4.71 ± 0.33 µm. The histomorphology of monocytes has an irregular shape, the cytoplasm is not granular and the nucleus is found hollow. Monocytes have an area of 22.59±5.09 µm2, perimeter of 16.69±2.37 µm, and diameter of 5.19±0.66 µm. Further research is needed on the histomorphometry of granulocytes: neutrophils, basophils, and eosinophils of Bali cattle.
HISTOLOGICAL STRUCTURE OF BALI DUCKS SKIN IN GROWER PHASE Dionysius Immanuel; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; I Ketut Berata; Luh Gde Sri Surya Heryani; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p07

Abstract

The skin is the largest and outermost organ that can be directly observed. The skin plays a crucial role for bali ducks as a protective barrier against the environment, maintaining body temperature, retaining body fluids, and neurosensory functions. This study aims to determine the structure of the bali duck skin (Anas sp.) during the grower phase. This study used ten bali ducks aged 3 months. The histological structure was observed using a binocular light microscope. The histological structure was analyzed descriptively and qualitatively. The structure of bali duck skin consists of three layers: epidermis, dermis, and hypodermis. The epidermis has three layers: stratum corneum, stratum intermedium, and stratum basale. The dermis has two layers: stratum compactum and stratum laxum. The tissues found in the bali duck skin include feather follicles, blood vessels, Herbst corpuscles, connective tissue, and musculus erector pili. Based on the research conducted, no differences in the histological structure of the skin were found across the three regions. Further research is needed on the skin structure of Bali ducks at different phases and sexes to obtain more comprehensive data, and specific histological studies are required to identify the skin histology of Bali ducks in greater detail.
QUALITY OF BOAR SEMEN EXTENDED IN PALM WATER-EGG YOLK DILUENT SUPPLEMENTED WITH A. DORSATA AND TRIGONA SP. HONEY Maria Patrisila Naibina; Wayan Bebas; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun; Desak Nyoman Dewi Indira Laksmi; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p27

Abstract

One of the factors that determine the success of artificial insemination in pig livestock is the quality of the semen used. The use of fresh semen without going through the dilution process will result in a decrease in the quality of spermatozoa. This study aims to determine the quality of Landrace pig semen in egg yolk palm water dilution with the addition of Apis dorsata and Trigona Sp. bee honey with a concentration of 3% each. This study used a complete random design pattern with five treatment groups, namely Beltsville Thawing Solution (BTS) diluent as a control (P0), palm fruit water diluent (P1), egg yolk palm water diluent (P2), egg yolk palm water diluent plus A. dorsata honey (P3) and egg yolk palm water diluent plus Trigona Sp. honey (P4). The diluted cement is stored at 150C for 48 hours. The observed semen quality parameters are motility, abnormality, viability, intact plasma membrane and intact acrosome membrane. The results showed that the water semen dilution of egg yolk palm fruit with the addition of A. dorsata honey with a concentration of 3% resulted in the highest motility, viability, intact plasma membrane and intact acrosome membrane and the lowest spermatozoa abnormalities when compared to Trigona Sp. honey and other treatments. It can be concluded that the egg yolk palm fruit water dilutor with the addition of A. dorsata honey with a concentration of 3% is able to maintain the quality of Landrace pig spermatozoa stored at 150C for 48 hours. Therefore, it is necessary to conduct further research on the fertility and litter size of Landrace pigs that are inseminating with egg yolk palm fruit water diluent with the addition of A. dorsata honey with a concentration of 3%.
Co-Authors Anak Agung Sagung Kendran Anak Agung Sagung Kendran Delima Marsinta Ida Pasaribu Desak Nyoman Dewi Indira Laksmi Dionysius Immanuel Gusti Agung Ayu Alecia Putri Anugrahaeni I Gde Andhika Putra Pratama I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Jagad Anom Ksatriya I Kadek Ari Satria Prayoga I Ketut Berata I Ketut Berata I Ketut Suatha I Nengah Wandia I Nyoman Suartha Ida Bagus Made Oka Luh Gde Sri Surya Heryani Luh Gde Sri Surya Heryani Luh Made Sudimartini Maria Patrisila Naibina Ni Ketut Suwiti Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Eka Setiasih Putri Utami Sri Kayati Widyastuti Sri Kayati Widyastuti Tjok Gde Oka Pemayun Wayan Bebas