THE EXTENDED STORAGE OF DILUTED CEMANI ROOSTER SEMEN USING EGG YOLK PHOSPHATE WITH THE ADDITION OF SUGARCANE JUICE

I Made Wahyu Chandra Bhadreswara (Mahasiswa Sarjana Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234)
Wayan Bebas (Laboratorium Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia)
I Wayan Sukernayasa (Laboratorium Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia)

Buletin Veteriner Udayana
Bul. Vet. Udayana. April 2025 Vol. 17 No. 2

Enhancing the population of Cemani chickens, a native Indonesian poultry genetic resource, requires reproductive technologies such as artificial insemination (AI). This study aimed to analyze the effect of storage duration on Cemani rooster semen diluted with a 20% egg yolk phosphate and 15% sugarcane juice formulation. A Completely Randomized Design (CRD) was employed with four storage periods (0, 24, 48, and 72 hours) at 5–7°C. Parameters observed included sperm motility, viability, and abnormalities. Results showed a decline in progressive motility from 80.33% (0 hours) to 54% (72 hours), viability from 88.17% (0 hours) to 62% (72 hours), and an increase in abnormalities from 5.17% to 9.33%. Despite the decline, all parameters remained within AI standards (motility >40%, abnormalities <20%) up to 72 hours. This study concludes that egg yolk phosphate and sugarcane juice combination is effective as a low-cost natural diluent for Cemani semen preservation. Further research is recommended to optimize diluent composition for longer storage.