This study aimed to evaluate the effectiveness of goat and sheep urine and determine the optimal dosage for alfalfa seedling growth. The experimental design used was a randomized complete factorial design consisting of two factors. Factor A is the urine type (goat or sheep), while factor B is the concentration of urine (5, 10, 15, 20, 25, 30, or 35%) + AB mix 400 ppm. The positive control consisted of 800 ppm AB mixture fertilizer, whereas the negative control received 0 ppm AB mixture. Each treatment was replicated 30 times. The results revealed a significant interaction effect (p < 0.05) between different types and levels of urine on media pH, plant height, and canopy biomass. Furthermore, the single factor of the urine level had a significant effect (p < 0.05) on the number of leaves. The results suggest that unfermented sheep urine liquid fertilizer at a level of 5% + AB mixture at 400 ppm is effective for alfalfa (Medicago sativa) seedlings. A relatively high urine level (? 10%) decreases the growth of plant vegetative tissue and has the opportunity to produce an abnormal leaf color (pale green).
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