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Ability of fiber bacteria isolated from buffalo rumen in digesting various sources of forage Prihantoro, Iwan; Toharmat, T; Evvyernie, D; ., Suryani; Abdullah, L
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1447.415 KB) | DOI: 10.14334/jitv.v17i3.699

Abstract

Local buffalo rumen fluid is a source of fiber digesting bacteria. Such bacteria presumably are well adapted to feed stuffs derived from agricultural byproducts with low quality. The purposes of this study were: (1) to isolate the fiber-digesting bacteria from buffalo rumen fluid, (2) to determine the fiber digesting characteristics and adaptability of such bacteria, and (3) to characterize the bacterial diversity.  Rumen fluids for the experiment were collected from buffaloes slaughtered at the Faculty of Animal Science, Bogor Agricultural University abattoir. Fiber-digesting bacteria were isolated using McDougall buffer supplemented with fibrous feed. A factorial experiment was conducted to study characteristics and adaptability of the bacteria using two factors: the type of bacteria and type of feed source of the fiber. Diversity among the bacteria was analyzed using the NTSys2.10 program.Results showed that nine bacterial isolates had a high adaptability to fiber feed based on CMCase. The highest CMCase activity bacteria for Pennisetum purpureum were A9 (11.36±1.70 unit/ml/h), A3 (11.22±0.60 unit/ml/h) and A42 (10.62±1.96 unit/ml/h). CMCase activity of fiber digesting bacteria from buffalo rumen fluid was not correlated with the number of bacteria in the culture. Based on genetic similarity, nine isolates were grouped into five types having similarity≥46%. Key Words: Buffalo Rumen Fluid, Fiber-Digesting Bacteria, Low Quality Forage
Productivity of duckweed (Lemna minor) as alternative forage feed for livestock in different light intensities Nopriani, Uti; Karti, PDMH; Prihantoro, Iwan
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 4 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.212 KB) | DOI: 10.14334/jitv.v19i4.1095

Abstract

Duckweed (Lemna minor) is a small aquatic plant that grow and float in water and spread extensively. Lemna minor is potential as a source of high quality forage. This study aimed to determine optimal light intensity on Lemna minor to generate maximum productivity. Parameters observed were physical-biological and chemical characteristics of the media (pH value, temperature, cover area, decreased of media volume, BOD, COD, nitrate, nitrite and phosphate), plant growth acceleration (number of shoots, leaf diameter and chlorophyll-a), biomass production, doubling time of cover area and the number of daughters. This study was done based on a completely randomized design with 4 levels of shading. While treatment was: without shading, shading 30%, shading 50% and shading 70% using paranet shade. Each treatment consisted of 4 replications. Result showed that the productivity of Lemna minor included the number of daughters, chlorophyll-a, biomass production, cover area, absorbed phosphate and doubling time the number of daughters reached the highest level without shading treatment (1007,21-2813,57 lux). The decrease of intensity of light, the increase the diameter of leaf. Decrease of media volume was positively correlated to size of cover area. Biomass production influenced by a wide doubling time of cover area and number of daughters. Key Words: Lemna minor, Light Intensity, Media, Productivity
Potency of fiber rumen bacterial isolates from local buffalo inoculated into Frisian Holstein calves during preweaning period Prihantoro, Iwan; Evvyernie, D; ., Suryani; Abdullah, L; Yunitasari, N.S; Sari, A.P; Khairunisa, D; Haziq, A; Rahayu, N; Toharmat, T
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.773 KB) | DOI: 10.14334/jitv.v17i4.696

Abstract

Fiber-digesting bacteria are the main rumen bacteria that play an important role in digesting feed. These bacteria are adapted to low quality forage from agricultural byproduct. The aim of these study was to determine the potency of fiber-digesting bacteria consortium obtained from buffalo rumen inoculated to Frisian Holstein calves during preweaning on feed consumption, utilization, mineral uptake and physiological status. This study used 14 isolates of bacteria obtained from collection of Faculty of Animal Science, Bogor Agricultural University. The experimental unit consisted of six Frisian Holstein calves at two week old with the average body weight of 38.00 ± 6.23 kg. Calves were inoculated by 20 ml of fiber-digesting rumen bacterial isolates [4.56 x 109 cfu/ml] every morning for four weeks. Experimental design used was based on a completly randomized design with three calves received the respective inoculation (treatment group) and three calves without any inoculation (control group). Data were analyzed statistically using t-test method with α = 0.05 and 0.01. The results showed that fiber-digesting bacteria (FDB) from rumen buffalo have adapted in the calves rumen since preweaning periode. Inoculation FDB increased the number of rumen bacteria, digestibility of protein and P uptake calves at eight weeks old. Increased feed intake, uptake of Mg and cobalt calves at 14 weeks old. Without causing any negative effects on ADG, physiological status and rumen fermentability. Key Words: Fiber-Digesting Bacteria, Nutrient Intake, Rumen Fermentability
Production and quality of Murdannia bracteata biomass as impact of magnesium foliar fertilizer Rahmawati, Tenti; Abdullah, Luki; Prihantoro, Iwan
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 3 (2015): SEPTEMBER 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.305 KB) | DOI: 10.14334/jitv.v20i3.1188

Abstract

Murdannia bracteata is one of potential forages for ruminant that has not been studied yet. This forage contents high mineral and it can be as fungtional feed. The aim of this study was to evaluate the growth and productivity, chlorophyll and minerals content of Murdannia bracteata caused by magnesium foliar application. The experiment was arranged in randomized complete design with five treatments and 4 replications. The application of magnesium level was arranged into: 0 ppm, 2000 ppm, 4000 ppm, 8000 ppm, and 12000 ppm. The study was conducted in a greenhouse, field laboratory of Agrostology, Faculty of Animal Science, Bogor Agricultural University. The result showed that growth, productivity, and chlorophyll content were not significantly (P>0.05) affected by increasing magnesium level. Increasing magnesium dossage resulted in decreasing calsium, potassium, and zink content (P<0,05), but not on phosphor and sodium (P>0,05). In conclusion, increasing magnesium dossage up to 12000 ppm did not significantly affect growth, productivity, and chlorophyll content. However, increasing magnesium level decreased calsium, potassium, and zink content. Key Words: Murdannia Bracteata, Magnesium, Productivity, Quality
Grazing Behavior and Itineraries of Kacang Goat with Different Coat Color under Semi Intensive Management Kiswanto, Slamet Heri; Baihaqi, Muhammad; Prihantoro, Iwan
ANIMAL PRODUCTION Vol 17, No 2 (2015): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (784.56 KB) | DOI: 10.20884/1.anprod.2015.17.2.541

Abstract

The objective of this research was to analyze the effect of coat color on behavior and itineraries of kacang goat during grazing time. This research used 9 females and 3 males kacang goat. Behavior observed by one zero sampling method and analyzed using t-Test at level 5%. The result indicated that ingestion and browsing of brown goat (30.91±2.87%; 8.75±3.10%) higher than black goat (28.57±2.69%; 6.07±4.78%), while black goat showed more locomotion (33.26±4.50%) than brown goat (29.70±4.63%). Grazing, panting, and resting behaviors, and distance traveled of black goat (22.56±2.63%; 4.48±4.02%; 2.34±2.97%; 483.48±133.16 m) were not different with brown goat (22.16±2.90%; 4.59±3.71%; 2.64±1.52%; 392.29±81.19 m). Result also  indicated that goat showed more grazing than browsing with high preference in you ng grass than old grass,  legume, and weed.
EKSPLORASI TUMBUHAN DI BAWAH NAUNGAN UBIKAYU SERTA POTENSINYA SEBAGAI HIJAUAN PAKAN Qomariah, Novia; Setiana, M Agus; Prihantoro, Iwan
Agros Journal of Agriculture Science Vol 16, No 1: Edisi Januari 2014
Publisher : Faculty of Agriculture, Janabadra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.295 KB)

Abstract

Pemanfaatan hijauan pakan ternak di antara Ubikayu belum optimal. Padahal potensinya sangat besar. Tujuan penelitian: mengeksplorasi dan mengidentifikasikan jenis hijauan pakan di bawah naungan ubikayu dan potensinya sebagai hijauan pakan. Penelitian berlangsung Maret 2014 di Komplek IPB Sinar Sari Dramaga. Metode penelitian: menetapkan area ubikayu sebagai perlakuan, yaitu ubikayu yang ditanam terpisah dengan tanaman lain (monokultur) dan yang ditanam di antara tanaman hortikultura dan palawija (polikultur). Selanjutnya diamati dan diambil sampel hijauan pakan di kedua lokasi lalu dibuat herbarium dan diidentifikasi jenisnya. Hasil: hijauan pakan yang tumbuh diantara tanaman Ubikayu monokultur lebih beragam dibandingkan dengan polikultur. Jenis-jenis hijauan pakan ternak yang tumbuh pada tanaman Ubikayu monokultur adalah Echinochloa colona, Setaria barbata, Famili Juncaceae, Cyperus sp., Paspalum conjugatum, Cynodon dactylon, Stenotaphrum secundatum, Axonophus compressus (Swartz) P. Beauv, Eleusine indica dan Panicum maximum. Jenis-jenis hijauan pakan ternak yang tumbuh pada tanaman Ubikayu polikultur adalah Echinochloa colona, Setaria barbata, Famili Juncaceae, Cyperus sp., Stenotaphrum secundatum, Eleusine indica dan Leucaena leucephala.
Kecukupan Nutrien dan Prevalensi Parasit Cacing pada Sapi Bali di Lahan Gambut Adrial Adrial; Rudy Priyanto; Salundik Salundik; Iwan Prihantoro
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.346 KB) | DOI: 10.18343/jipi.25.2.270

Abstract

Peatlands have specific characteristics with waterlogged conditions, fragile, and less fertile. The level of peatlands fertility dependent on depth peat and the distance from rivers. This study was aimed to evaluate nutrient adequacy and prevalence of worm infestations in the gastrointestinal tract of Bali cattle that maintained in different peatland categories. The research was conducted in Pulang Pisau District, Central Kalimantan. The site was determined by purposive sampling and divided into 3 categories peat swamp, shallow peat near the river, and shallow peat away from the river. The data were collected by survey method and laboratory analysis. The variables observed included forage quality, nutrient intake, the number of infected cows, and the number of eggs per gram of feces (EPG). The results showed that each category of peatland have different feed quality and nutrient adequacy (P<0.05). The nutrient intake in the peat swamps was relatively higher than that of other sites. In general, the nutrient adequacy of cattle raised in the peatlands area was still below the standard requirement. In all peatland categories, the occurring of Ca deficiency in the diet was pronounced. The highest prevalence of parasitic worms infection was found in cows raised in peat swamps. Bali cattle that maintained in different peatland categories generally showed different responses in nutrient adequacy and prevalence of parasitic worms infection. Keywords: Bali cattle, nutrient adequacy, parasitic worm, peatlands, prevalence
Studi Pengembangan Jagung Berkelanjutan melalui Integrasi dengan Sapi di Tuban, Jawa Timur Suwarto Suwarto; Iwan Prihantoro
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.418 KB) | DOI: 10.18343/jipi.25.2.232

Abstract

Biji-bijian dan daging adalah dua komoditas penting di Indonesia. Jawa Timur adalah sentra produksi utama kedua komoditas tersebut sehingga perlu diusahakan keberlanjutannya. Permasalahan dalam produksi jagung adalah peningkatan harga input pupuk anorganik dan keterbatasan ketersediaan bahan tersebut. Permasalahan produksi ternak adalah keterbatasan pakan. Biomas batang, daun, kelobot, dan tongkol sebagai produk samping jagung dapat digunakan menjadi pakan ternak. Sebaliknya, kotoran sapi dapat digunakan sebagai sumber pupuk. Integrasi kedua sistem ini menjadi peluang untuk keberlanjutan produksi. Studi ini dilakukan untuk mengetahui keberlanjutan produksi jagung dan sapi di Tuban, Jawa Timur melalui penentuan skala dan tingkat keterpaduan integrasi. Survei terhadap 23 orang petani anggota Kelompok Tani Makaryo, Desa Waleran, Kecamatan Grabagan dilakukan untuk mengetahui pengusahaan jagung dan sapi. Untuk mengetahui efisiensi pemupukan, di lahan kelompok tani dilakukan penelitian dengan menggunakan empat dosis urea, yaitu 0, 100, 200, dan 300 kg/ha, dengan pupuk dasar 300 kg NPK Phonska dan 10 ton pupuk kandang. Skala integrasi yang dapat dilaksanakan oleh satu rumah tangga petani adalah 1 ha jagung dengan 2 ekor sapi dengan tingkat keterpaduan ekologis 1,1; ternak sapi memasok pupuk kandang untuk 1,1 ha kebun jagung. Penggunaan urea sebanyak 200 kg menghasilkan biji sebanyak 6.890 kg dan penerimaan tertinggi sebesar Rp21.288.000. Urea dihemat 100 kg ha-1 atau 9.651 ton untuk luas tanaman jagung 96.505 ha/tahun di Tuban. Secara ekonomis, integrasi peternakan sapi dengan kebun jagung dapat menghemat input luar sebanyak Rp230.000/ha atau Rp20.651 miliar/tahun. Pertanian jagung yang terintegrasi dengan sapi dapat mewujudkan pertanian berkelanjutan bermasukan luar rendah. Kata kunci: bermasukan luar rendah, biomas, integrasi, pakan, pupuk kandang
Anaerobic fermentation effectively reduces concentration of total tannins in Chromolaena odorata Yelly M. Mullik; Muhammad Ridla; Iwan Prihantoro; Marthen L. Mullik
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.731 KB) | DOI: 10.14334/jitv.v21i1.1301

Abstract

Chromolaena odorata is a potential feed source but its usage is hampered by presence of various secondary metabolic compounds in plant’s tissues. One group of them is tannin. This experiment was aimed to evaluate various pre-treatment methods on total tannin concentration and in vitro digestibility of dry- and organic-matter. An 8 x 3 completely randomized experimental design was employed to test 8 different treatments. The treatments were: Fresh = freshly-chopped chromolaena  leaves as control,  Sun-dried = sun-dried (3x 24 hours) chromolaena leaves, Oven-dried = oven-dried (60oC for 24 hours) chromolaena leaves,  Boiled = water-boiled (5 minutes) chromolaena leaves, RenWater = water-soaked (4 hours) chromolaena leaves,  RenNaOH = NaOH-soaked (4 hours) chromolaena leaves,  RenHCl = HCl-soaked (4 hours) chromolaena leaves, and Fermented = anaerobically-fermented (21 days) chromolaena leaves. Parameters measured were concentration of total tannins and nutrient content. The results showed that application of low heat (Sun-dried), hot water (Boiled), water soaking (RenWater), or unaeobic fermentation technique significantly reduced total tannin by 43% into 62% compared to control. The highest suppression (62%) was achieved by Fermented treatment. In the contrary, medium heat application (oven-dried at 60°C) or chemicaltreatments (HCl or NaOH) had no effect. Protein content of chromolaena was improved by 60% and crude fiber was reduced by 32% in Fermentaed treatment compared to the control. It could be concluded that anaerobic fermentation can be used an effective strategy to reduce tannin concentration in Chromolaena odorata without affecting its feeding value.
Growth response of leucaena embryogenic callus on embryo age differences and Auxin 2,4-Dichlorophenoxyacetic acid Satria Julier Manpaki; Iwan Prihantoro; Panca Dewi M.H. Karti
Jurnal Ilmu Ternak dan Veteriner Vol 23, No 2 (2018): JUNE 2018
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.186 KB) | DOI: 10.14334/jitv.v23i2.1538

Abstract

Leucaena (Leucaena leucocephala cv. Tarramba) is a source of protein from the legume family. Tarramba varieties able to adapt well in tropical area such as West Nusa Tenggara and East Nusa Tenggara. This study aimed to identify the growth response and embryogenic callus morphology of lamtoro (L. leucaena cv. Tarramba) in embryos different ages and auxin 2,4-D levels. This research was used explants derived from lamtoro (L. leucocephala cv. Tarramba) as much 400 explants. this study conducted of 16 treatments with two factors, the first factor is the provision of PGR 2,4-D concentration of 0.5 mg L-1, 1 mg L-1, 1.5 mg L-1 and 2 mg L-1. The second factor was the type of embryo such as mature embryo, cotyledon, heart, and pre-globular. Each treatment was repeated 25 replications. This study uses a randomized complete design (CRD) with two factor. Data were analyzed using analysis of variance and if there was significant difference, data were further analyzed using Duncan’s multiple range test. Analysis of variance showed that PGR 2,4-D at a concentration of 1.5 mg L-1 and the type of optimum embryo was mature embryo callus on parameters as height, diameter increment callus, and callus color scores were significantly different (P <0.05). callus texture parameter indicates results that are uniform throughout the treatment that was compact callus. Lamtoro plant embryogenic callus (L. leucocephala cv. Tarramba) indicate an optimal response at the concentration of PGR 2,4-D 1.5 mg L-1 and the type of embryo was mature embryo.