This study aims to analyze the development of studies on the integration of ethnoscience into biology learning in high schools during the 2021–2025 period. The method used was a systematic literature review (SLR) by searching articles through Google Scholar using keywords related to ethnoscience and biology education in high schools. A total of 30 articles met the inclusion and exclusion criteria and were analyzed through a process of identifying, reviewing, analyzing, and interpreting data. The results showed that the majority of studies focused on the development of ethnoscience-based learning tools (36.7%) and their implementation in the classroom (33.3%). In the domain of learning outcomes, the greatest improvement occurred in critical thinking skills (33.3%), followed by cognitive skills, scientific literacy, understanding of scientific concepts, and psychomotor skills. The material most frequently associated with local wisdom was biodiversity (29%). These findings confirm that ethnoscience is effective in strengthening conceptual understanding and higher-order thinking skills through a culturally relevant context.
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