Mosquito control as the primary vector of Dengue Hemorrhagic Fever (DHF) transmission can be achieved by breaking the transmission chain through vector population management at the larval stage. Larval control can utilize biolarvicides from natural materials, including crude extract of citronella (Cymbopogon nardus). This study aimed to examine the effectiveness of citronella extract as a larvicide against Aedes aegypti larvae instar III and IV. This experimental research employed a Completely Randomized Design (CRD) with larvae treated using extract concentrations of 2%, 2.5%, 3%, 3.5%, and 4.5% in 250 mL of water. The control group used sterile distilled water without extract. Mortality observations were conducted at 1, 3, 6, 12, 24, and 48 hours with four replications. Mortality percentage data were analyzed using probit analysis to determine LC₅₀ and LT₅₀ values. The results indicated that citronella extract was effective in killing 50% of the test larvae population with an LC₅₀ of 36.48% and an LT₅₀ of 10.45 hours.
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