SRIWIJAYA JOURNAL OF ENVIRONMENT
Vol 10, No 3 (2025): ENVIRONMENTAL ENGINEERING

Antibacterial Activity of Lactic Acid Bacteria Isolated from Cincalok Against Escherichia coli and Listeria monocytogenes

Oktavia, Yulia (Unknown)
Lubis, Zuwita Cahaya Rani Br (Unknown)
Amrizal, Sri Novalina (Unknown)
Wulandari, Wulandari (Unknown)



Article Info

Publish Date
28 Jan 2026

Abstract

This study aims to determine the antibacterial activity of lactic acid bacteria (LAB) isolates from cincalok, a traditional shrimp fermentation product, against Escherichia coli and Listeria monocytogenes. Five lactic acid bacteria isolates (A1, A2, A3, A4, and A5) were assayed for their antagonistic activity against E. coli and L. monocytogenes. The antagonistic assays were conducted with different incubation times of the LAB isolates before the antagonistic assays. The incubation times were 18, 21, and 24 hours. LAB isolates that showed inhibition zones were further assayed in antibacterial assays. Antibacterial assays were conducted by the disk diffusion method. Antagonistic and antibacterial assays were each carried out in triplicate. The antagonistic assay results showed that only isolates A3 and A4, which were incubated for 24 hours, showed inhibition zones. Inhibition zones of isolate A3 against L. monocytogenes were 1.33 ± 1.04 mm, while against E. coli were 0.67 ± 0.29 mm. Inhibition zones of isolate A4 against L. monocytogenes were 1.33 ± 1.04 mm, while against E. coli were 1 ± 0.5 mm. Antibacterial assay results showed inhibition zones of isolate A3 against L. monocytogenes were 4.67 ± 0.58 mm, while E. coli 6.67 ± 2.08 mm. Inhibition zones of isolate A4 against L. monocytogenes were 3.67 ± 0.58 mm, while E. coli 4.33 ± 0.58 mm. Antibacterial activity of Isolates A3 and A4 had weak inhibition against L. monocytogenes and E. coli. Antibacterial activity depended on the type of target bacteria and was influenced by incubation time and environmental conditions.

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Journal Info

Abbrev

ppsunsri

Publisher

Subject

Agriculture, Biological Sciences & Forestry Energy Environmental Science Public Health

Description

Sriwijaya Journal of Environment (SJE) publishes original research or theoretical papers, notes, and mini reviews on new knowledge and research or research applications on current issues in environmental sciences and related such as: Environmental Science, Environmental Technology, Environmental ...