This study is motivated by several issues, namely the low interest of students in biotechnology, the lack of understanding of basic concepts of biotechnology among students, the difficulty of implementing biotechnology practicum directly in schools, and the absence of E-Student Worksheets used in biotechnology materials by teachers. The purpose of this study is to determine the feasibility and effectiveness of an E-Student Worksheet based on project based learning on biotechnology material. The method employed in this study is research and development, with the developed product being an E-Student Worksheet based on PJBL on biotechnology material using the ADDIE model, which consists of five stages: Analyze, Design, Development, Implementation, and Evaluation. The results indicate that product validation by material experts achieved a percentage of 80.00% in the feasible category, while validation by media experts reached 82.5% in the very feasible category. Teacher responses to the product showed a percentage of 86.66% in the very good category, and student responses yielded 87.73% in the very interesting category for the individual trial, 90.18% in the very interesting category for the small group trial, and 92.55% in the very interesting category for the limited group trial. The effectiveness of the E-Student Worksheet was obtained with a percentage of 92.21% in the very effective category. In conclusion, the E-Student Worksheet based on project-based learning on biotechnology material can be used to improve students' psychomotor learning outcomes
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