Medula
Vol 2, No 2 (2015)

Perbandingan Metode Proporsi dengan Metode Resazurin Microtiter Assay (Rema) untuk Deteksi Mycobacterium tuberculosis yang Resisten Terhadap Rifampisin

Yenti Purnamasari (Unknown)
Sartini Sartini (Unknown)
Faisal Attamimi (Unknown)
Muh. Nasrum Massi (Unknown)



Article Info

Publish Date
08 Jul 2017

Abstract

Tuberculosis (TB) is one of the high infectious diseases in Indonesia caused by Mycobacteriumtuberculosis. Drugs which used for tuberculosis therapy has been widely reported experiencing resistance, while inappropriately treatment will lead to not effective and efficient care. Culture in Lowenstein Jensen medium is a gold standard to perform the drug susceptibility but it takes a long time to get results, therefore, new methods were developed to replace it, one of them is colorimetric method. This research aimed to compare Rifampicin susceptibility test in colorimetric method use REMA and culture method use Lowenstein Jensen medium. This study was a cross sectional with experimental laboratory design used 42 sputums from patients with tuberculosis, done at October 2010 until March 2011. In this research, 5 samples rifampicin resistant have been detected. As a results, we founded Rifampicin susceptibility test of REMA have 80 % sensitivity, 100 % specificity, 100 % PPV, and 97 % NPV when compared with culture method.Keywords:Proportion, Resazurin, Mycobacterium tuberculosis, Resistance

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Journal Info

Abbrev

medula

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Description

MEDULA mengundang kontribusi bagi penelitian original dan fundamental pada bidang kesehatan sebagai sebuah artikel yang melewati proses ...