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Yenti Purnamasari
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Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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Uji Aktivitas Antibakteri Ekstrak Etanol Bawang Putih (Allium sativum) terhadap Pertumbuhan Bakteri Salmonella typhii dan Shigella dysenteriae Gyidian Upa; Agusalim Ali; Yenti Purnamasari
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 4, No 2 (2017)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.141 KB) | DOI: 10.46496/medula.v4i2.2811

Abstract

ABSTRACT  Diseases of gastrointestinal infections, especially those caused by the bacterium Salmonella typhii and Shigella dysenteriae still a health problem in Indonesia. Garlic (Allium sativum) is a traditional medicine which has antibacterial properties. Garlic contains Allisin, alkaloids, tannins and saponins. The purpose of this study was to determine the antibacterial activity of ethanol extract of the bulbs of garlic (Allium sativum) on the growth of the bacteria Salmonella typhii and Shigella dysenteriae. This study is experimental with post test only control group design. Test of inhibition is determined based on the diameter of clear zone formed. Testing was performed against a pure extract of garlic, the positive control, negative control and solvent control for comparison. Each treatment is performed three times. The results showed that the ethanol extract of garlic (Allium sativum) does not produce a clear zone well against Salmonella typhi and Shigella dysenteriae, it can be concluded that the ethanol extract of garlic (Allium sativum) has no antibacterial activity against Salmonella typhii and Shigella dysenteriae. It is recommended to use another method to extract further research as the development or continuation of this research Keywords: Antibacterial activity, garlic(Allium sativum), Salmonella typhi,  Shigella dysenteriae.
Acute Toxicity Test of Gedi (Abelmoschus manihot L.) Ethanol Leaves Extract to Histopatology of Liver Mice Mutmainna Mutmainna; Yenti Purnamasari; Parawansah Parawansah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 6, No 3 (2019): Edisi Suplemen
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.43 KB) | DOI: 10.46496/medula.v6i3.9651

Abstract

ABSTRACTBackground: Abelmoschus manihot L.are widely used  as a daily dish  of dishes and traditional medicine to treat various diseases. It contains alkaloids, triterpenoids, saponins, and flavonoids wich can be toxic agent at variable dose. Purpose: This study aimed determine the effect of ethanol extract of abelmoschus manihot L. on histopatologic image of mice liver tissue.  Methods: This is quasi experimental study  with post test only control group design. Fourty five mice  (mus musculus)  as the subject of this research, they divided into 5 groups, group I threated by 50 mg /kgBB extract, group II threated by 200 mg / kgBB extract, group III threated by 800 mg / kgBB extract, group IV threated by 3,200 mg / kgBB extract, and group V as negative control. Treatment was done for 14 days and terminated at next day for histopatology examination use them liver tissue. Result: Histopathological examination of liver cells within normal limits. In the treatment group of 50 mg liver tissue of mice who died during treatment on day 7 and day 10 showed liver tissue necrosis. In the treatment group of 200 mg and 800 mg of liver tissue showed the presence of liver cell necrosis, and cell boundaries appeared unclear. In the treatment group of 3200 mg liver tissue showed necrotic cells, lysi s cells, unclear  cell  boundaries  and  necrotic  cells  more  widely.Conclusion:  As  a  conclusion  there  an histopatologic changes of  microscopic examination of mice liver cells in the form of necrosis and, there is not significant difference in ALT and AST  between the control group.Keywords : Abelmoschus manihot L. extract, acute toxicity, liver histopathology
Uji Aktivitas Antibakteri Ekstrak Kasar Bawang Putih (Allium sativum ) terhadap Bakteri Shigella dysenteriae yang Resisten terhadap Ampisilin Arimaswati Arimaswati; Yessy Toding Mendaun; Yenti Purnamasari
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 6, No 2 (2019)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.378 KB) | DOI: 10.46496/medula.v6i2.6723

Abstract

ABSTRACT Background : Gastrointestinal infections, especially caused by Shigella dysenteriae bacteria is still a health issue in Indonesia. The number of ampicillin resistance to S. dysenteriae has been widely reported. Garlic (Allium sativum) is a traditional medicine that efficacy as an antibacterial. Garlic contains an organosulfur compound  Allicin. Research Purpose: This study aimed to determine the antibacterial activity of garlic rule extract on the growth of S. dysenteriae bacteria that are resistant to ampicillin. Research Methods: This study used the experimental with post test only control group design. The antibacterial activity test was determined based on the diameter of clear zone formed. Test  were performed  on garlic filtrate, ciprofloxacin as a positive control and aquades as a negative control. Each treatment was performed three times. Research Result: This research showed that rule extract of garlic (A. sativum)  had a clear zone against S. dysenteriae that was resistant to ampicillin. Conclusion: The conclusion of this research was that rule extract of garlic (A. sativum) had an antibacterial activity against S. dysenteriae which resistant to ampicillin.  Keywords : Antibacterial activity, Garlic (A. sativum), S. dysenteriae
Perbandingan Metode Proporsi dengan Metode Resazurin Microtiter Assay (Rema) untuk Deteksi Mycobacterium tuberculosis yang Resisten Terhadap Rifampisin Yenti Purnamasari; Sartini Sartini; Faisal Attamimi; Muh. Nasrum Massi
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 2, No 2 (2015)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.576 KB) | DOI: 10.46496/medula.v2i2.2548

Abstract

Tuberculosis (TB) is one of the high infectious diseases in Indonesia caused by Mycobacteriumtuberculosis. Drugs which used for tuberculosis therapy has been widely reported experiencing resistance, while inappropriately treatment will lead to not effective and efficient care. Culture in Lowenstein Jensen medium is a gold standard to perform the drug susceptibility but it takes a long time to get results, therefore, new methods were developed to replace it, one of them is colorimetric method. This research aimed to compare Rifampicin susceptibility test in colorimetric method use REMA and culture method use Lowenstein Jensen medium. This study was a cross sectional with experimental laboratory design used 42 sputums from patients with tuberculosis, done at October 2010 until March 2011. In this research, 5 samples rifampicin resistant have been detected. As a results, we founded Rifampicin susceptibility test of REMA have 80 % sensitivity, 100 % specificity, 100 % PPV, and 97 % NPV when compared with culture method.Keywords:Proportion, Resazurin, Mycobacterium tuberculosis, Resistance
Keragaman Mikroorganisme pada Swab Vagina Perempuan Menopause di Kota Kendari Tahun 2020 Yenti Purnamasari; Juminten Saimin; Sufiah Asri Mulyawati
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 8, No 2 (2021)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46496/medula.v8i2.20617

Abstract

ABSTRAKKetidakseimbangan mikrobiota vagina akibat berbagai perubahan fisiologis dan hormonal wanita pramenoupause tentunya akan mempengaruhi keberagaman flora pada lingkungan vagina. Flora mikroba dalam  vagina  bersifat  heterogen.  Keseimbangan  mikrobiota  pathogen  dan  non  patogen  dipengaruhi berapa faktor seperti keasaman, kelembaban dan lainnya dimana pada perempuan menopause sudah mulai terjadi perubahan. Telah dilakukan sebuah penelitian yang bertujuan untuk melihat gambaran mikrobiota pada swab vagina perempuan menopause di kota Kendari pada tahun 2020. Penelitian ini merupakan penelitian deskriptif dilakukan pada bulan Oktober hingga Desember 2020. Sebanyak 32 sampel swab vagina perempuan menopause dijadikan sampel padapenelitian ini. Terhadap keseluruhan sampel dilakukan pemeriksaan KOH serta kultur PDA dan SDA untuk melihat jamur, pewarnaan Giemsa untuk melihat parasit, kultur pada medium MC dan MSA untuk melihat bakteri. Dari hasil yang diperoleh ditemukan bakteri gram positif, bakteri gram negatif, jamur dan parasit. Perlu penelitian lebih lanjut untuk identifikasi mikrobiota yang didapatkan pada sampel penelitian ini. Kata Kunci: Mikroorganisme, Swab Vagina, Menopause.
Edukasi dan Pemeriksaan Kesehatan untuk Meningkatkan Kesadaran Masyarakat tentang Stroke di Wilayah Pesisir Toronipa, Soropia Ashaeryanto; Mulyawati, Sufiah Asri; Satrio Wicaksono; Sulastrianah; Muhammad Rustam HN; Yenti Purnamasari; Aan Maulana; Izur Fisabilillah Aminudin
Jurnal Pengabdian Meambo Vol. 4 No. 1 (2025): Jurnal Pengabdian Kepada Masyarakat MEAMBO
Publisher : PROMISE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56742/jpm.v4i1.103

Abstract

Stroke menjadi penyebab utama kematian di Indonesia, termasuk di Kelurahan Toronipa, Soropia. Rendahnya pemahaman masyarakat tentang faktor risiko seperti hipertensi dan gaya hidup tidak sehat diperburuk oleh faktor geografis dan keterbatasan akses layanan kesehatan. Pengabdian ini bertujuan untuk meningkatkan kesadaran masyarakat terhadap pencegahan stroke secara efektif. Kegiatan dilaksanakan tanggal 20 November 2024, di Kantor Kelurahan Toronipa. Pendekatan berbasis penyuluhan, edukasi, dan pemeriksaan kesehatan gratis, melibatkan tenaga medis, kader, dan tokoh masyarakat. Penyuluhan mencakup bahaya stroke, faktor risiko, dan deteksi gejala awal dengan metode FAST. Pemeriksaan kesehatan meliputi pengukuran tekanan darah, gula darah, kolesterol, dan konsultasi medis. Peserta berjumlah 50 orang. Hasil pengabdian menunjukkan bahwa mayoritas responden perempuan (74%), dengan risiko meningkat pasca-menopause. Kelompok dewasa (42%) dan pra lansia (38%) menjadi fokus pencegahan dini, sementara lansia (20%) memerlukan manajemen penyakit kronis. Faktor risiko utama meliputi kolesterol tinggi (38%), diabetes (10%), dan hipertensi (54%). Edukasi gaya hidup sehat efektif menurunkan risiko. Kesimpulan pengabdian adalah bahwa meningkatkan kesadaran pencegahan stroke melalui edukasi, deteksi dini, dan gaya hidup sehat, dengan dampak positif jangka panjang.
Co-Authors Aan Maulana Agusalim Ali Arimaswti Arimaswati Ashaeryanto Faisal Attamimi Gyidian Upa Izur Fisabilillah Aminudin Juminten Saimin Muh. Nasrum Massi Muhammad Rustam HN Mulyawati, Sufiah Asri Mutmainna Mutmainna Parawansah Parawansah Sartini Sartini Satrio Wicaksono Sulastrianah Yessy Toding Mendaun