Physical Stability Test and Total Phenolic Content Determination of Spray Gel Formulation with Ethanol 70% Melinjo Leaf (Gnetum gnemon L.) Extract with Combination of Carbopol 940 and HPMC as Gelling Agent

Nelly Suryani (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)
Vivi Anggia (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)
Halimah Dewi (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)
Rosa Adelina (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)
Sabrina Dahlizar (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)
Ofa Suzanti betha (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)
Estu Mahanani Dhilasari (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)
Ismiarni Komala (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)
Yardi Saibi (Department of Pharmacy, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia)

Pharmaceutical And Biomedical Sciences Journal (PBSJ)
Vol. 7 No. 2 (2025)

The stability of a pharmaceutical preparation is very important in formulation to ensure product efficacy and safety. This research aims to test the physical stability and total phenolic content of a spray gel preparation made from 70% ethanol extract of melinjo leaves (Gnetum gnemon L.) with a combination of Carbopol 940 and HPMC as gelling agents. Antioxidant activity was tested using the DPPH method, while total phenolic content was measured using the Folin-Ciocalteu reagent and expressed in gallic acid equivalents (mgGAE/g). The results analysis of total phenolic content in the 70% ethanol extract of melinjo leaves showed a value of 46.237 mg GAE/g. Meanwhile, the total phenolic content in the spray gel formulation of the 70% ethanol extract of melinjo leaves was recorded at 0.88 mg GAE/g."Based on evaluation, the best formula is a combination of Carbopol and Hidroxypropyl methylCellulosa, HPMC (0.4%:0.4%), but the stability test results show physical instability. Further modifications are required to produce a physically stable formula.