This research explores the influence of the Problem Based Learning approach on students’ comprehension of studying the human digestive system in Biology at the high school level. This study used a quantitative correlational approach with 30 students chosen by applying simple random sampling. The data were obtained through a behavior a rating scale questionnaire to measure PBL implementation and multiple-choice tests to assess comprehension. The results show that PBL increases active participation, critical thinking, and problem-solving, leading to better understanding of the digestive system. The analysis reveals that PBL contributes about 69% to students’ comprehension, with the rest being because of other factors. To conclude, PBL significantly improves learning outcomes and is recommended as a successful approach to teaching Biology.
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