<![CDATA[Problems that often arise in the process of freezing semen is destruction of the plasma membrane because the sperm membrane contains unsaturated fatty acids are highly susceptible to peroxidation damage. α-tocopherol is an antioxidant that reduce damage due to peroxidation. The purpose of the study was to determine α-tocopherol antioxidant supplementation in diluter on plasma membrane integrity after freezing process. Semen was collected using artificial vagina from goat aged 2 to 2.5 years in normal reproduction. The design used was randomized block design with treatment dose of a different α-tocopherol (0 grams, 0.2 grams, 0.4 grams, 0.6 grams) in 100 ml of extender. The results showed that the percentage to plasma membrane integrity equilibrated at a dose of 0.4 g (75.46%) was higher (P <0.05) compared with a dose of 0.0 g (73.78%), and the dose of 0.6 g (72.93%), but did not differ (P> 0.05 ) with a dose of 0.2 g (75.46%). Plasma membrane integrity examination results at post thawing at dose of 0.4 g (73.16%)was higher (P <0.05) than a dose of 0.0 g (69.8%), 0.2 g (71.53%), 0.6 g (70.7 %) respectively. It was concluded that supplementation of antioxidant α-tocopherol 0.4 ml is the best dose to maintain membrane integrity of semen frozen of goat.]]>
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