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Analisis litter size, bobot lahir dan bobot sapih hasil perkawinan kawin alami dan inseminasi buatan kambing Boer dan Peranakan Etawah (PE) Kaunang, Dedy; Suyadi, S.; Wahjuningsih, Sri
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 23, No 3 (2013)
Publisher : Faculty of Animal Science, Universitas Brawijaya

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ABSTRACT:  The quality of semen inseminated to the dam might influence on the fer-tilization process and fetal growth during pregnancy. The aim of this research was to analysis the litter size, birth and weaning weight of Boer x PE goat crosses. Forty one parous and multiparous Peranakan Etawah (PE) does were naturally mated with selected Boer buck (NM-group), while the other 24 does were inseminated using artificial tech-nique using semen collected from the same buck as NM-group  and called as AI-group. The result showed no significant differences in litter size, birth weight and weaning weight between kids resulting from the does mated naturally and artificially insemina-tion technique (1.8±0.64 vs. 1.79±0.58; 2.93±0.568 vs. 2.96±0.397 kg; and 11.06 ± 2.001 kg vs. 11.31 ± 1.54 kg, for litter size, birth weight and weaning weight, respec-tively. It was concluded that mating technique in cross mating between PE does x Boer buck did not influence on the litter size and early growth phase of kids. Keywords: PE does, Boer buck, natural mating, artificial insemination
Pengaruh pengencer Tris Aminomethane kuning telur yang disuplementasi sari kulit Manggis (Garcinia Mangostana) terhadap kualitas semen Sapi Limousin selama penyimpanan suhu dingin 50C Effendi, Fredyan Isnan; Wahjuningsih, Sri; Ihsan, M. Nur
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 25, No 3 (2015)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2015.025.03.09

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The purpose of this research was to determine the dose of mangosteen rinds juice (0%, 2%, and 4%) in a diluent Tris Aminomethane-egg yolk. The fresh semen was collected once a week using an artificial vagina from five Limousin bulls aged 8 to 11 years old with a body weight of 895-1034 kg. The research method was experimental laboratory using a randomized block design with 3 treatments and 10 replications. If there were significant influences among the variables, it would be then tested by Duncan’s Multiple Range test method. The result showed that Tris Aminomethane-egg yolk diluent with 4% of mangosteen rinds juice suplementation had significant differences (P<0.05) on the sperm quality kept for 72 hours in the cold storage in terms of the percentage of sperm motility and sperm viability. It also had a significant difference (P<0.05) on sperm abnormality particularly within the 1st hour. It can be concluded that the addition of 4% mangosteen rinds juice in Tris Aminomethane-egg yolk diluent could protect Limousin bull semen quality (motility, viability and abnormality) after stored for 72 hours at 50C. Keywords: Abnormality, liquid semen, mangosteen rind, motility, viability
Relationship between body condition score on the service per conception and conception rate of Brahman Cross cows Ervandi, Mohamad; Ihsan, Moh. Nur; Wahjuningsih, Sri; Anugra Yekti, Aulia Puspita; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 1 (2020): April 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.01.08

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The factors that influence the success of Artificial Insemination is affected by acceptors that will be used for AI which have a good Body Condition Score, because BCS has a relationship with animal reproduction, such as fertility and pregnancy. The purpose of this research was to evaluate and determine the relationship of Body Condition Score (BCS) on Service per Conception (S/C) and Conception Rate (CR). The material used was 80 Brahman Cross (BX) cows, the sample was selected by Purposive Sampling with the criteria 2-3 years old with body weight ranged from 300-500 kg, gave birth, and BCS between 4-7 (scale 1-9). The method used in this study was the survey method. The variables observed were the relationship between Body Condition Score (BCS), Service per conception (S/C), and Conception Rate (CR). The data was obtained by using correlation analysis and also a simple regression with the SPSS 2.0 software program, then analyzed descriptively. The results showed that the equation of relationship between BCS and S/C variables obtained r = 0.961% and the regression value equation y = -25.998 + 1.520x (R2) 23.9%. The relationship between BCS and CR variables obtained a value of r = 0.976% and BCS regression equation value with CR y = -39.727 + 1.109x (R2) 29.5%. The research thus concludes that there is a positive relationship that is being between BCS on the S/C and CR of Brahman Cross cows. 
Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage Wahjuningsih, Sri; Wulandari, Desinta
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 3 (2019): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.03.08

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The success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage (3-5ºC). The semen was collected from Boer goat aged 2.5 to 3 years old by using an artificial vagina. The semen with >2+ mass motility and >70% individual motility were then selected for the study. A randomized block design which consisted of four treatments with ten replications as follow: P0 or control (CEP-3 + 10% egg yolk + 0.4% albumen); P1 (CEP-3 + 10% egg yolk + 0.4% albumen + 1% WCE; P2 (CEP-3 + 10% egg yolk + 0.4% albumen + 2% WCE); P3 (CEP-3 + 10% egg yolk + 0.4% albumen + 3% WCE) was used in this study. The data were analyzed with ANOVA and followed with Duncan’s multiple range test to determine significant differences. The results of this study showed that motility, viability, and membrane integrity in all treatments showed highly significant differences (P<0.01), while no significant difference (P>0.05) was shown on the semen abnormality. The result concludes that 1% WCE supplementation to the CEP-3 extender showed the best Boer semen quality during 96-hours cold storage. 
Interaction Effect of Vitamin E-selenium Supplementation and Metabolic Energy on Reproductive Performance of Joper Breeders Nining Haryuni; Hartutik Hartutik; Eko Widodo; Sri Wahjuningsih
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2842

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The side effect of fatty acid oxidation during lippoprotein synthesis is the release of oxygen in the tissue called reactive oxygen species (ROS). Metabolic stress in Joper brooders due to an imbalance between ROS and antioxidants causes a decrease in hatching egg production and quality. Therefore, research is needed to improve the reproductive performance of Joper broodstock by combining vitamin E-selenium supplementation and metabolic energy. This study used 200 hatched eggs resulting from a cross between 60 weeks old Sentul males and 35 weeks old ISA BROWN laying hens that had been treated. This study used a Factorial Completely Randomized Design (CRD) (2 x 4). The first factor is the energy level (2700 and 2800 kcal/kg) and the second factor is the dose of vitamin E-selenium supplementation (0, 25, 50, 75 and 100 ppm). Selenium dosage is 1ppm/mg vitamin E. The interaction between vitamin E-selenium supplementation and energy had a very significant effect (P<0.01) in increasing DOC weight. The single factor energy level and vitamin E-selenium supplementation significantly (p<0.05) in reducing embryo mortality and increasing hatchability, while fertility and eggshell quality were not affected by the two treatment factors. The conclusion of this study is the interaction between vitamin E-selenium supplementation and energy levels can increase the weight of Joper's DOC, while the single factor of vitamin E-selenium supplementation and energy levels can reduce embryo mortality and increase hatchability. The best interaction with 100 ppm vitamin E-selenium supplementation and energy 2800 kcal/kg.
Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing Sri Wahjuningsih
Journal of Innovation and Applied Technology Vol 4, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.699 KB) | DOI: 10.21776/ub.jiat.004.01.7

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Problems that often arise in the process of freezing semen is  destruction of the plasma membrane because the sperm membrane contains unsaturated fatty acids are highly susceptible to peroxidation damage. α-tocopherol is an antioxidant that  reduce damage due to peroxidation. The purpose of the study was to determine α-tocopherol antioxidant supplementation in diluter on   plasma membrane integrity after freezing process. Semen was collected using artificial vagina from  goat aged  2 to 2.5 years in normal reproduction. The design used was randomized block design with treatment dose of a different α-tocopherol (0 grams, 0.2 grams, 0.4 grams, 0.6 grams) in 100 ml of extender. The results showed that the percentage  to   plasma membrane integrity equilibrated at a dose of 0.4 g (75.46%) was higher (P <0.05) compared with a dose of 0.0 g (73.78%), and the dose of 0.6 g (72.93%), but did not differ (P> 0.05 ) with a dose of 0.2 g (75.46%).  Plasma membrane integrity  examination results at  post thawing  at dose of 0.4 g (73.16%)was  higher (P <0.05) than a dose of 0.0 g (69.8%), 0.2 g (71.53%), 0.6 g (70.7 %) respectively. It was concluded that supplementation of antioxidant α-tocopherol 0.4 ml is the best dose to maintain  membrane integrity of semen frozen of goat.
PENGARUH PENGGUNAAN CEP-2 DENGAN SUPLEMENTASI KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI FH (FRISIAN HOLSTEIN) KUALITAS RENDAH SELAMA PENYIMPANAN SUHU 4-5OC Dedi Muhammad; Trinil Susilawati; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 1 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.558 KB) | DOI: 10.21776/ub.jtapro.2016.017.01.8

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Tujuan dari penelitihan ini adalah pengaruh penggunaan CEP-2 dengan suplementasi kuning telur pada berbagai level terhadap kualitas spermatozoa sapi FH kualitas rendah selama penyimpanan pada suhu 4-5 oC. Penelitian ini dilakukan di Laboratorium Reproduksi Ternak Fakultas Peternakan Universitas Brawijaya, tanggal 17 September hingga 18 November 2014. Metodologi yang digunakan adalah percoboaan menggunakan rancangan acak kelompok (RAK). Penelitihan ini menggunakan 3 perlakukan, yaitu (P1 = CEP-2 + 10% Kuning Telur, P2 = CEP-2 + 15% Kuning Telur, P3 = CEP-2 + 20% Kuning Telur) dengan 10 ulangan. Hasil penelitihan menunjukkan bahwa pengencer CEP-2 dengan suplementasi 10%, 15%, 20% kuning telur tidak berpengaruh secara signifikan (P> 0,05) terhadap persentase motilitas, viabilitas dan abnormalitas spermatozoa sapi FH yang memiliki kulitas rendah. Semen segar kualitas rendah dalam pengencer CEP-2 yang ditambahkan 10%, 15% dan 20% kuning telur dapat bertahan sampai hari ke 1 untuk dapat digunakan dalam proses IB. Disarankan untuk memanfaatkan semen segar sapi FH yang memiliki kualitas rendah untuk inseminasi buatan.Kata kunci: semen, pengencer, motilitas, viabilitas, abnormalitas.
PENGARUH PENAMBAHAN GLUTATHIONE PADA PENGENCER TRIS AMINOMETHANE KUNING TELUR DALAM MEMPERTAHANKAN KUALITAS SPERMATOZOA SAPI LIMOUSIN SELAMA PENYIMPANAN SUHU RUANG Rahman Maulana; Nurul Isnaini; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 1 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.442 KB) | DOI: 10.21776/ub.jtapro.2016.017.01.7

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Penelitian ini dilaksanakan di Balai Besar Inseminasi Buatan (BBIB) Singosari pada bulan Maret sampai April 2016. Tujuan penelitian untuk mengetahui pengaruh pemberian glutathione pada pegencer Tris Aminomethane kuning telur dalam mempertahankan kualitas semen sapi Limousin selama penyimpanan suhu ruang. Materi yang digunakan adalah semen segar dengan motilitas individu 45-55%. Metode penelitian yang digunakan adalah percobaan. Metode percobaan dengan menggunakan Rancangan Acak Kelompok dan dianalisis dengan menggunakan analisis ragam. Perlakuan pada penelitian ini P0 ( 0 mM glutathione), P1 (0,5 mM glutathione), P2 (1 mM glutathione) dan P3 (1,25 mM glutathione) dengan 10 ulangan. Hasil penelitian menyatakan bahwa penambahan glutathione pada pengencer Tris Aminomethane kuning telur berbeda sangat nyata (P<0,01) persentase motilitas individu selama penyimpanan 3 dan 24 jam, sedangkan viabilitas dan abnormalitas spermatozoa pada semua perlakuan tidak berbeda nyata (P>0,05). Penyimpanan semen 3 jam dan 24 jam disuhu ruang sebaiknya menggunakan penambahan 0,75 mM glutathione dalam Tris Aminomethane kuning telur agar mempertahankan motilitas spermatozoa.Kata kunci : abnormalitas, Motilitas, Pengencer,semen, viabilitas
KUALITAS SEMEN SAPI LIMOUSIN PADA PENGENCER YANG BERBEDA SELAMA PENDINGINAN Veronica Devita Bunga; Trinil Susilawati; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 15, No 1 (2014): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.863 KB)

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This study was done to evaluate quality of Limousin’s semen during chilled preservation. The characteristic of spermatozoa were easily damaged, so that they were  necessary to protect viability of spermatozoa. Addition of extender was conducted to determine quality and viability of spermatozoa. Each extender were added Egg Yolk as diluents cryopreservation, the treatment were devided in to three groups, they were ; P0 (CEP-2 + 10% Egg Yolk), P1 (Tris Aminomethane + 20% EggYolk), P2 (Skim + 10% Egg Yolk). The result showed that Tris Aminomethane + 20% Egg Yolk gave the best effect on maintaining quality of spermatozoa (motility, viability and abnormality). The motility in three extender at 24h was high significant different (P<0,01). Furthemore, the Viability and abnormality showed high significant different (P<0,01) since first time evaluation at 0h until 24h. Tris Aminomethane showed the best result among others due to the addition of 20% Egg Yolk, as we know that Egg Yolk was an extracellular cryoprotectan to protect and nourish spermatozoa during chilling preservation.   Keyword : Egg Yolk, CEP-2, Tris Aminomethane, Skim,
KUALITAS SEMEN SEGAR DAN RECOVERY RATE (RR) SAPI LIMOUSIN PADA MUSIM YANG BERBEDA Siti Sunami; Nurul Isnaini; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.431 KB) | DOI: 10.21776/ub.jtapro.2017.018.01.6

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This research aimed to determine the quality of fresh semen and recovery rate (RR) Limousin bulls at different seasons in Indonesia. The research materials were used semen sample and recording of 10 Limousin bulls on November 2015 until September 2016. The analyze was used by descriptive, while seasonal factors were determined by rainfall, they were divided into 2 stage (summer and rainy). The result was showed that the average of individual motility (60±0.07 %) on summer and (57.7±0.09 %) on rainy, Consentration of spermatozoa (1043±208 million/ml) on summer and (1013±175 million/ml) on rainy, post thawing motility (44±0.02 %) on summer and (41±0.05 %) on rainy, RR value (65±0.05 %) on summer and (55±0.09 %) on rainy, the production of frozen semen (1979±644.2 straw) on summer and (1623±379 straw) on rainy. It was concluded that the best quality of fresh until frozen semen was generally on summer with low rainfall. The seasonal effect for quality of semen Limousin bulls were individual motility, concentration, PTM, and RR value will be impacted for frozen semen produce. It was advisable to rejected the Limousin bulls with poor semen quality.Keywords: Seasonality, Summer, Rainy, Quality of Semen