The transformation of learning in the era of artificial intelligence requires students to develop strong digital literacy skills accompanied by ethical awareness in technology use. This study examines the implementation of digital literacy and the challenges of integrating AI ethics in Biology learning at MAN 2 Kota Malang. A descriptive approach was employed, with data collected through interviews with a Biology teacher and an AI digital literacy questionnaire administered to 60 tenth-grade students. The questionnaire assessed students’ critical understanding, awareness of social impacts, ability to use AI, and ethical behavior. The findings indicate that students demonstrate relatively strong proficiency in using digital technologies and AI for learning. However, they still encounter difficulties in higher-order skills, such as critically evaluating information, identifying bias, and reflecting on the ethical implications of AI use. Teachers have introduced instructional innovations involving AI, augmented reality, and virtual reality, yet challenges remain in pedagogical integration, ethical guidance, and students’ readiness. These findings highlight the need to strengthen value-based AI digital literacy in order to foster reflective awareness, ethical judgment, and academic integrity, thereby enabling Biology learning to move beyond technological efficiency toward more meaningful and responsible educational practices.
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