This study aims to determine the validity of the design of an E-Booklet on Hydrocarbonoclastic Bacteria in Petroleum Wastewater for biodiversity material for Grade X senior high school students. The research method used was descriptive qualitative with the ADDIE model consisting of five stages, namely Analyze, Design, Development, Implement, and Evaluate; however, the E-Booklet design in this study was carried out only up to the Development stage. The E-Booklet design stage was conducted at the Biology Study Program, Faculty of Teacher Training and Education, Bina Widya Campus, Universitas Riau. The designed E-Booklet was then validated by two validators, namely a media expert and a content expert. The validators’ assessment of the E-Booklet was based on E-Booklet validity indicators consisting of three aspects: content, language, and format and graphics. The validity results of the E-Booklet design on Hydrocarbonoclastic Bacteria in Petroleum Wastewater obtained an average validity score of 3.84, indicating that the E-Booklet design falls into the very valid category and can be used as enrichment teaching material for biodiversity content for Grade X senior high school, Phase E.
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