<![CDATA[The widespread emergence of antimicrobial resistance has intensified the search for plant-derived antibacterial agents as therapeutic alternatives. Soursop (Annona muricata L.) is widely recognised in Indonesian ethnomedicine as a plant effective against infections, owing to its rich and diverse secondary metabolite profile. This study was designed to examine the growth-inhibitory capacity of the ethyl acetate leaf fraction of soursop against pathogenic bacteria Staphylococcus aureus and Escherichia coli in vitro, while concurrently identifying the fraction concentration producing the greatest inhibitory effect. Antibacterial testing employed the agar diffusion disc technique at three fraction concentrations: 10%, 20%, and 40% (w/v). The resulting inhibition zone diameters were subjected to one-way ANOVA and Duncan's Multiple Range Test. All concentrations successfully suppressed the growth of both test organisms; the maximum inhibition was recorded at 40%, with mean inhibition zone diameters of 4.49 mm for S. aureus and 4.06 mm for E. coli. Statistical analysis confirmed a highly significant treatment effect (Fcount = 21.02; p<0.01). Accordingly, the ethyl acetate fraction of soursop leaves demonstrates dose-dependent antibacterial potential worthy of further development.]]>
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