<![CDATA[Background: Antioxidants are compounds that play a role in inhibiting or reducing oxidative damage caused by free radicals. Objective: This study aimed to evaluate the characteristics of simplicia, phytochemical constituents, reflux extraction process, and antioxidant activity of puguntano herb extract using the DPPH method with UV-Vis spectrophotometry. Methods: Simplicia characterization included macroscopic examination, moisture content, water-soluble extractive value, ethanol-soluble extractive value, and total ash content. Phytochemical screening was performed using thin-layer chromatography to detect alkaloids, flavonoids, saponins, tannins, glycosides, and steroids/triterpenoids. Puguntano herb was extracted by reflux using n-hexane, ethyl acetate, and methanol as solvents, followed by concentration with a rotary evaporator to obtain thick extracts. Antioxidant activity was assessed using the DPPH method after 60 minutes of incubation at a wavelength of 516 nm. Results: The simplicia characterization showed a moisture content of 7.23%, water-soluble extractive value of 38.6%, ethanol-soluble extractive value of 5%, and total ash content of 10.71%. Phytochemical screening revealed the presence of flavonoids, saponins, tannins, glycosides, and steroids/triterpenoids. Conclusion: The IC50 values of the n-hexane, ethyl acetate, methanol extracts, and quercetin were 216.8478, 30.6781, 21.3478, and 4.973 µg/mL, respectively]]>
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