Indonesian Journal of Biotechnology
Vol 17, No 2 (2012)

Selection of Yeast Strains for Ethanol Fermentation of Glucose-FructoseSucrose Mixture

J. Jasman (Doctoral Program of Biotechnology, Graduate School of Universitas Gadjah Mada, Yogyakarta, Indonesia Department of Natural Sciences Education, Universitas Nusa Cendana, Kupang, Indonesia)
Irfan Dwidya Prijambada (Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Chusnul Hidayat (Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Donny Widianto (Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia)



Article Info

Publish Date
06 Dec 2012

Abstract

This study was aimed to compare the ability of some yeast strains to consume sugars (sucrose, glucoseand fructose) and to convert them into ethanol during fermentation. The results of this comparison will be thebasis of considerations in choosing the right strain to be used as a mixed culture to increase the productionof ethanol from substrate containing a mixture of sucrose, glucose and fructose, such as juice of cane andsweet sorghum. The study was conducted using fermentation in substrate consisting of glucose, fructose,and sucrose separately, glucose-fructose mixture, and glucose-fructose-sucrose mixture using some yeaststrains: FNCC3012, OUT7009, OUT7027, OUT7055, OUT7080, OUT7096, OUT7903, OUT7913, and OUT7921.Following the fermentation, analysis of the produced ethanol and the remaining sugar was conducted. Theresults of study indicated that the strains with the highest substrate consumption were OUT7921, OUT7096,OUT7055, OUT7027, and OUT7913 for glucose, fructose, glucose-fructose mixture, sucrose, and glucosefructose-sucrose mixture, respectively. Strains that produced highest concentration ethanol were OUT7096 inglucose and sucrose substrates, OUT7921 in substrate of glucose-fructose mixture and sucrose, OUT7913 insubstrate of glucose-fructose-sucrose mixture. Upon consideration of each strain capacity, both in consumingsugar and producing ethanol, the recommended strains for use in mixed culture in bioethanol fermentationusing mixed substrate of glucose, fructose and sucrose are OUT7096, OUT7913, and OUT7921.

Copyrights © 2012






Journal Info

Abbrev

ijbiotech

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...