<![CDATA[Nuts have the potential to lower blood sugar levels and increase insulin sensitivity in people with diabetes mellitus through antioxidant, anti-inflammatory, and α-glucosidase enzyme inhibition activities due to their phytochemical and melatonin content. Melatonin is known to lower blood sugar through the mechanism of inhibiting the α-glucosidase enzyme, but there has been no research related to the melatonin content in local Indonesian nuts. This study aims to identify phytochemicals and melatonin and test the α-glucosidase enzyme inhibitory activity in extracts of red beans, peanuts, soybeans, tolo beans, and mung beans. Samples were prepared in the form of simples, then extracted by maceration method. Qualitative identification of phytochemicals (flavonoids, phenolics, alkaloids, tannins, and saponins) was carried out using specific reagents, while total flavonoid and total phenolic levels were determined quantitatively by UV-Vis spectrophotometry. Melatonin content was tested using Thin Layer Chromatography (TLC), and α-glucosidase enzyme inhibitory activity was tested in vitro. Data analysis was performed using linear regression and One-Way ANOVA. The results showed that all extracts contained flavonoids, phenolics, and alkaloids, with saponins found only in soybeans. The highest flavonoid and phenolic content was found in peanuts, while the best α-glucosidase inhibitory activity was demonstrated by red beans with an IC50 value of 0.4057 mg/mL.]]>
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