Jurnal Riset Kefarmasian Indonesia
Vol. 8 No. 2 (2026): Jurnal Riset Kefarmasian Indonesia

Analysis of Antioxidants and Flavonoid Compounds of Ethanol Extract of Starfruit Leaves (Averrhoa Bilimbi L) Using the DPPH Method

Ersalina Nidianti (Analis Kesehatan, Fakultas Kesehatan, Universitas Nahdlatul Ulama Surabaya)
Widi Agustin Wulandari (Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya)
Dina Kamelia (Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya)



Article Info

Publish Date
30 May 2026

Abstract

Starfruit (Averrhoa bilimbi L.) is not only a commonly consumed fruit but also widely used in traditional medicine. The plant has long been utilized by the community to help treat various ailments, including coughs and rectal inflammation. This study aimed to determine the total flavonoid content and antioxidant activity of ethanol extracts of starfruit leaves that had been fractionated using n-hexane.The extraction process was carried out using the maceration method with 70% ethanol, followed by fractionation with 95% n-hexane to separate active compounds from the crude extract. The total flavonoid content in the n-hexane fraction of the ethanol extract was measured using a UV-Vis spectrophotometer at a wavelength of 229-230 nm, while antioxidant activity was evaluated using the DPPH method, also measured at 515 nm. The results showed that the total flavonoid content of the n-hexane fraction of the ethanol extract was 37,3 mgQE/g extract. The antioxidant activity test yielded an IC50 value of 6.58 μg/mL, compared to a standard IC50 value of 2.45 μg/mL for vitamin C. These findings indicate that the n-hexane fraction of starfruit leaf ethanol extract possesses very strong antioxidant properties.

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Journal Info

Abbrev

jrki

Publisher

Subject

Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Description

JURNAL RISET KEFARMASIAN INDONESIA adalah jurnal yang diterbitkan online dan diterbitkan dalam bentuk cetak. Jurnal ini diterbitkan 3 kali dalam 1 tahun (Januari, Mei dan September) Jurnal ini diterbitkan oleh APDFI (Asosiasi Pendidikan Diploma Farmasi Indonesia) Lingkup jurnal ini mencakup ...