Jurnal Ilmu Kefarmasian Indonesia
Vol. 24 No. 1 (2026): JIFI

Validation of HPLC for identification of captopril, enalapril, and lisinopril in traditional Indonesian herbal medicine

Liliek Nurhidayati (Departemen of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia)
Mawarni Tobing (Indonesian Food and Drug Authority Regional Office in Jakarta, Jakarta, 13870, Indonesia)
Elvi Rahmayuni (Indonesian Food and Drug Authority Regional Office in Jakarta, Jakarta, 13870, Indonesia)



Article Info

Publish Date
30 Apr 2026

Abstract

Traditional Indonesian herbal medicine (Jamu) is widely used for health maintenance and disease  prevention. The addition of medicinal chemicals to Jamu is not in accordance with quality and safety requirements. The purpose of this study was to develop a valid HPLC method for the identification of captopril, lisinopril, and enalapril in Jamu for hypertension. In this study, they were identified using high-performance liquid chromatography with a photodiode array detector. The stationary phase was a C18 YMC column (length 250 mm, diameter 4.6 mm, particle size 5 μm), methanol - 0.1 % phosphoric acid (45:55) as the mobile phase with isocratic elution, a flow rate of 1.0 mL/min, a column temperature of 40 °C, an injection volume of 20 μL, and detection at  210 nm. The results showed that this method fulfilled the specificity requirement, with detection limits of captopril, enalapril, and lisinopril of 0.715, 0.795, and 1.403 ppm, respectively. This HPLC method can be used for the identification of captopril, enalapril, and lisinopril in commercial Jamu.

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Journal Info

Abbrev

jifi

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

Description

Jurnal Ilmu Kefarmasian Indonesia (JIFI) mainly focuses on a current topic in Pharmaceutical Sciences are also considered for publication by the Journal. Discussions on a topic in Pharmaceutical Sciences, Clinical Sciences, and Social Behaviour Administration. Detailed scopes of articles accepted ...