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EFEKTIVITAS DAUN CINCAU HIJAU (Cyclea barbata Miers) SEBAGAI ANTIOKSIDAN ALAMI PADA PRODUK JAMBAL PATIN (Pangasius hypopthalmus) Farida Ariyani; Nova Susanti Saputri; Liliek Nurhidayati
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 2 (2009): Desember 2009
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v4i2.449

Produk perikanan yang berlemak tinggi seperti jambal patin sangat rentan mengalami kemunduran mutu karena oksidasi. Daun cincau hijau dikenal mengandung senyawa antioksidan alami. Untuk menghambat kemunduran mutu ikan karena oksidasi, serangkaian percobaan menggunakan ekstrak daun cincau hijau dilakukan dengan merendam patin asin dalam ekstrak daun cincau hijau pada konsentrasi 0%; 0,5%; 1,0%; dan 1,5% selama 30 menit sebelum pengeringan. Sebagai kontrol positif digunakan perendaman dalam Butylated Hydroxy Toluene (BHT) pada konsentrasi 0,1%, sedangkan untuk kontrol negatif digunakan patin asin tanpa penambahan ekstrak daun cincau hijau. Pengukuran bilangan Thiobarbituric acid (FBA), produk berfluoresen (fluorescent products), dan penilaian organoleptik dilakukan selama penyimpanan pada suhu kamar, sedangkan pengukuran profil asam lemak tidak jenuh dilakukan pada jambal patin sebelum penyimpanan. Hasil penelitian menunjukkan bahwa perlakuan paling efektif dalam memperlambat proses oksidasi dan dapat diterima panelis adalah perendaman patin asin dalam ekstrak cincau hijau pada konsentrasi 0,5% selama 30 menit dengan nilai TBA 2,0-2,9 µmol/kg ikan, produk berfluoresen 0,21-0,24 µg/g ikan, dan asam lemak tidak jenuh 5,6%.

EFEKTIVITAS DAUN CINCAU HIJAU (Cyclea barbata Miers) SEBAGAI ANTIOKSIDAN ALAMI PADA PRODUK JAMBAL PATIN (Pangasius hypopthalmus) Farida Ariyani; Nova Susanti Saputri; Liliek Nurhidayati
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 2 (2009): Desember 2009
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v4i2.449

Produk perikanan yang berlemak tinggi seperti jambal patin sangat rentan mengalami kemunduran mutu karena oksidasi. Daun cincau hijau dikenal mengandung senyawa antioksidan alami. Untuk menghambat kemunduran mutu ikan karena oksidasi, serangkaian percobaan menggunakan ekstrak daun cincau hijau dilakukan dengan merendam patin asin dalam ekstrak daun cincau hijau pada konsentrasi 0%; 0,5%; 1,0%; dan 1,5% selama 30 menit sebelum pengeringan. Sebagai kontrol positif digunakan perendaman dalam Butylated Hydroxy Toluene (BHT) pada konsentrasi 0,1%, sedangkan untuk kontrol negatif digunakan patin asin tanpa penambahan ekstrak daun cincau hijau. Pengukuran bilangan Thiobarbituric acid (FBA), produk berfluoresen (fluorescent products), dan penilaian organoleptik dilakukan selama penyimpanan pada suhu kamar, sedangkan pengukuran profil asam lemak tidak jenuh dilakukan pada jambal patin sebelum penyimpanan. Hasil penelitian menunjukkan bahwa perlakuan paling efektif dalam memperlambat proses oksidasi dan dapat diterima panelis adalah perendaman patin asin dalam ekstrak cincau hijau pada konsentrasi 0,5% selama 30 menit dengan nilai TBA 2,0-2,9 µmol/kg ikan, produk berfluoresen 0,21-0,24 µg/g ikan, dan asam lemak tidak jenuh 5,6%.

Specificity of Mercury Detecting Reagents for Skin Lightening Creams Liliek Nurhidayati; Titiek Martati; I Wayan Redja; Nofanti Sandra
JURNAL ILMU KEFARMASIAN INDONESIA Vol 16 No 1 (2018): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Inorganic mercury compound have been used for long time in cosmetics preparations for its skin lightening effect. The used of mercury in cosmetics has been prohibited as stipulated in the decision of the BPOM RI (Head of the Food and Drug Supervisory Agency) No.HK.00.01.432.6147. Based on health department research from various countries, there are a number of skin lightening products containing mercury between 660-57.000 μg/kg. People needs a quick and easy way to detect mercury in a lightening cream, so that people can test the safety of cream that used. One alternative that can be used to detect mercury in skin lightening cosmetics is using detecting reagents. The aim of this study was preparation of specific and sensitive mercury detecting reagents. The detecting reagents consisted mixtures of diphenylcarbazone and acetic acid in 96% of ethanol. The specificity test is performed by adding diphenylcarbazone solution into a lightening cream containing whitening agents such as mercury, hydroquinone, bangkuang extract and mixture of these substances. Assay was also performed on simulated cream that contained mercury, zinc oxide, bismuthsubnitrate, and mixture of mercury, zinc oxide and bismuthsubnitrate. The mercury detecting reagents gives a distinctive result that shown by the formation of purple mercury complex in less than 3 minutes.

Optimization of Extract Concentration and Polyvinyl Pirolidon Binding Material on Granule Red Betel Leaves Extract (Piper crocatum Ruiz & Pav) as Antihyperuricemic Dian Ratih Laksmitawati; Liliek Nurhidayati; Mochamad Futuchul Arifin; Bagus Bahtiar
JURNAL ILMU KEFARMASIAN INDONESIA Vol 15 No 2 (2017): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Ethanolic extract of red betel leaves (Piper crocatum Ruiz & Pav) has antihyperuricemic activity. In order to be applicable to consume, this extract need to be formulated into pharmaceutical dosage form. Granules which is packed in a tea sac bag was considered to be more practical andappropriate for person suff ering hyperuricemic, because they have to drink more water. In the granulation process, the he binder plays an important role so that this study aims to fi nd the optimal red betel leaf granule formula with variation on the extract dose and the polyvinyl pyrolidone (PVP) binder and prove granule activity in vivo in mouse hyperuricemia model. The extract was prepared by macerationthe leaves in 96% ethanol and sprayed dried by hot air. The powder was then formulated to granules form in 22 factorial design. The factors were the extract dosages (38% dan 47.51%) dan the amount of PVP (Polyvinyl Pyrrolidone) as a binder (0.5% dan 2%). The physical and chemical properties of the granules were evaluated, interaction dosage, PVP concentration and each properties was analysed by making the contour plot and superimposed contour plot to get the optimum formula. The in vivo antihyperuricemic activity of the granules was determined in hyperuricemic mice. The result showed that the optimum formula was found in granules that contained 42.5% extract and 1% PVP. At the equivalent dosage of 455 mg extract/kg, this granules formula had antihyperuricemic activity decreasing 54.2 %plasma uric acid level significantly. Thus it can be concluded that the optimum granules of ethanol extract of red betelleaf with 42.5% extra ct content and 1% PVP proved to have antihiperurisemia activity in mice induced by chicken extractand potassium oxonate.

Sifat Fisikokimia dan Aktivitas Antioksidan Crude Fukoidan Hasil Ekstraksi dari Sargassum cinereum Liliek Nurhidayati; Yashinta Fitriani; Syamsudin Abdillah; Esti Mumpuni; Mohamad Rafi
JURNAL ILMU KEFARMASIAN INDONESIA Vol 18 No 1 (2020): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Brown seaweed has been known as a fucoidan source that has various biological activities. Fucoidan could be obtained by various extraction methods. This study aims to extract, characterize and to evaluate the antioxidant activity of crude fucoidan extracted from Sargassum cinereum. Extraction was carried out by refluxing at 100oC for 4 and 5 hours and then precipitated with ethanol. The crude fucoidan was brown powder. The yield with 4 hours extraction time was 2.78% which did not differ significantly from the 5 hours extraction time. Phytochemical screening of crude extract showed that beside fucoidan there were flavonoids, saponins, steroids, triterpenoids, and tannins. The extraction with duration of 4 hours and 5 hours had sulfate content of 16.31% and 11.22%, total carbohydrate content were 22.94% and 22.85%, and in the FTIR spectrum showed OH absorption bands of carbohydrates, fucose, sulfates and uronic acid. Testing of antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhyrazyl) free radical inhibition method produced IC50 values of 721.9 µg/ml and 749.9 µg/ml respectively and it was concluded that crude fucoidan of Sargassum cinereum had weak antioxidant activity.

Spektofotometri Derivatif dan Aplikasinya dalam Bidang Farmasi Liliek Nurhidayati
JURNAL ILMU KEFARMASIAN INDONESIA Vol 5 No 2 (2007): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1078.4 KB)

Derivative spectrophotometry is a method for the simultaneous determination of drugs mixtures due to their overlapping spectra. Since the practical introduction of the concept of spectral data derivatization, the derivative spectrophotometry technique was applied. This review describes the theoretical and instrumental aspects of derivative spectrophotometry and some of its applications in pharmaceutical analysis since 1999.

Efek Antioksidan Larutan Kosolven Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) pada Tikus dengan Parameter MDA dan SOD ROS SUMARNY; LILIEK NURHIDAYATI; SITI SOFIAH; YATI SUMIYATI; FRANSISKA DIANA SANTI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 1 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.637 KB)

Mangosteen (Garcinia mangostana L.) rinds extract in capsule form has been marketed. a-mangostin, the active compound of G. mangostana had been known has low solubility in water. This study was aimed to measure and compare antioxidant effect of a-mangostin as cosolvent solution to extracts suspentions. 25 rats were divided into 5 groups: negative (I) and positive (II) control, cosolvent solution (III), extract (IV) and extract® (V). Treatment were given for 14 days, free radicals were induced by CCl . MDA were analyzed using thiobarbituric acid method while SOD by using adenochrome assay. Results showed MDA level were: 1.0±0.19; 0.3±0.05; 0.4±0.04; 0.6±0.04 and 0.5±0.00 nmol/mL, whereas SOD activity were: 17±0.0; 123±25.3; 107±19.0; 73±18.9 and 97±27.0 U/mL for group I, II, III, IV and V, respectively. Kruskal Wallis test showed a significant difference in MDA between group III with IV and V (p>0.05), showing cosolvent has higher ability to decrease MDA than the extracts suspension. There were significant differences of SOD between group III and IV (p>0.05), meaning cosolvent has higher ability to increase SOD activity compare to the extracts. Research showed cosolvent has a higher antioxidant effect with potential to inhibit MDA formation by 70% and increase SOD activity by 623%.

High Performance Liquid Chromatography Method Validation Of α-Mangostin Assay in Mangosteen (Garcinia mangostana L.) Fruit Rind Extract Formulated in Oral Solution Liliek Nurhidayati; Siti Sofiah; Ros Sumarny; Kevin Caesar
ALCHEMY Jurnal Penelitian Kimia Vol 11, No 1 (2015): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.11.1.104.38-46

Mangosteen fruit rind extract contain a lot of antioxidants. α-Mangostin is a component in mangosteen fruit rind that has highest antioxidant effect. The oral solution containing mangosteen fruit rind extract is required an assay method for quality assessment. Determination of a very low concentration of analyte in sample with very complex matrix, such as α-mangostin in oral solution, needs a selective and sensitive method, such as high performance liquid chromatography (HPLC). In this study, α-mangostin assay was performed by reverse phase HPLC system using octadecylsilane (C18) as stationary phase, methanol-water (90:10) as mobile phase, the flow rate is 1.0 mL/min, and the UV detector at 316 nm. The retention time of α-mangostin was 9.622 minutes. Peak of α-mangostin was well separated with resolution of 1.725. Linearity was in the range of 1.67-5.01 ppm with correlation coefficient of 0.9986. The relative standard deviation (RSD) was 1.30 %, the recovery was in the range of 95.80-100.76

High Performance Liquid Chromatography Method Validation Of α-Mangostin Assay in Mangosteen (Garcinia mangostana L.) Fruit Rind Extract Formulated in Oral Solution Liliek Nurhidayati; Siti Sofiah; Ros Sumarny; Kevin Caesar
ALCHEMY Jurnal Penelitian Kimia Vol 11, No 1 (2015): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.11.1.104.38-46

Mangosteen fruit rind extract contain a lot of antioxidants. α-Mangostin is a component in mangosteen fruit rind that has highest antioxidant effect. The oral solution containing mangosteen fruit rind extract is required an assay method for quality assessment. Determination of a very low concentration of analyte in sample with very complex matrix, such as α-mangostin in oral solution, needs a selective and sensitive method, such as high performance liquid chromatography (HPLC). In this study, α-mangostin assay was performed by reverse phase HPLC system using octadecylsilane (C18) as stationary phase, methanol-water (90:10) as mobile phase, the flow rate is 1.0 mL/min, and the UV detector at 316 nm. The retention time of α-mangostin was 9.622 minutes. Peak of α-mangostin was well separated with resolution of 1.725. Linearity was in the range of 1.67-5.01 ppm with correlation coefficient of 0.9986. The relative standard deviation (RSD) was 1.30 %, the recovery was in the range of 95.80-100.76

High Performance Liquid Chromatography Method Validation Of α-Mangostin Assay in Mangosteen (Garcinia mangostana L.) Fruit Rind Extract Formulated in Oral Solution Liliek Nurhidayati; Siti Sofiah; Ros Sumarny; Kevin Caesar
ALCHEMY Jurnal Penelitian Kimia Vol 11, No 1 (2015): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.11.1.104.38-46

Mangosteen fruit rind extract contain a lot of antioxidants. α-Mangostin is a component in mangosteen fruit rind that has highest antioxidant effect. The oral solution containing mangosteen fruit rind extract is required an assay method for quality assessment. Determination of a very low concentration of analyte in sample with very complex matrix, such as α-mangostin in oral solution, needs a selective and sensitive method, such as high performance liquid chromatography (HPLC). In this study, α-mangostin assay was performed by reverse phase HPLC system using octadecylsilane (C18) as stationary phase, methanol-water (90:10) as mobile phase, the flow rate is 1.0 mL/min, and the UV detector at 316 nm. The retention time of α-mangostin was 9.622 minutes. Peak of α-mangostin was well separated with resolution of 1.725. Linearity was in the range of 1.67-5.01 ppm with correlation coefficient of 0.9986. The relative standard deviation (RSD) was 1.30 %, the recovery was in the range of 95.80-100.76

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