<![CDATA[Boesemani rainbowfish (Melanotaenia boesemani) is a freshwater ornamental fish with high economic value and is endemic to Papua Island. This species is widely favored due to its distinctive morphology and unique coloration, characterized by an elongated and laterally flattened body, with bright blue coloration toward the head and bright orange coloration toward the caudal region. Male Boesemani rainbowfish are particularly preferred because of their more attractive and varied coloration. Masculinization is a method used to increase the production of male fish. One natural material that can be applied for masculinization is honeybee propolis extract. This study was conducted at the Fish Reproduction Laboratory, Faculty of Fisheries and Marine Science, Brawijaya University, Malang, from December 2017 to January 2018. An experimental method using a Completely Randomized Design (CRD) was applied with four treatments and a control. The treatments consisted of immersion durations of 10, 12, 14, and 16 hours using a dose of 1 ppm. Data were analyzed using analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) test. The main parameters were the percentage of male fish and survival rate, while supporting parameters included growth rate and water quality. The highest percentage of males was obtained in treatment B (12 hours), reaching 62.35%, compared with 45.45%, 47.78%, 39.49%, and 11.82% in treatments A, C, D, and control, respectively. Survival rates ranged from 42.22% to 90%, while growth rates ranged from 0.0198 to 0.0211 g. Water quality remained within the observed ranges. The results indicate that immersion using propolis extract significantly influenced masculinization when applied at an optimal duration.]]>
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