Journal of Pharmaceuticals and Natural Sciences
Vol. 3 No. 2 (2026): J Pharm Nat Sci

Analysis of Rhodamine B in Lipsticks Marketed in Wonosobo, Indonesia, Using Rapid Test Kits and UV–Visible Spectrophotometry

Nova Andina (Unknown)
Dharmastuti Cahya Fatmarahmi (Universitas Muhammadiyah Yogyakarta)



Article Info

Publish Date
27 May 2026

Abstract

Rhodamine B is a synthetic dye that is commonly used as a colorant in the paper and textile industries. Rhodamine B is a hazardous dye that is frequently used as a colorant in cosmetics due to its relatively low cost and ability to produce more attractive colors. The use of Rhodamine B in cosmetics can be hazardous to health as it can cause cancer, respiratory tract irritation, eye irritation, skin irritation, and digestive disorders. The objective of this study is to determine the presence of Rhodamine B in lipsticks available on the market in the Wonosobo region. This study employed both qualitative and quantitative methods. The qualitative analysis in this study utilized a rapid test kit to quickly detect the presence of Rhodamine B in lipstick samples based on color changes resulting from the addition of reagents. Samples suspected of containing Rhodamine B were analyzed quantitatively using UV-Vis Spectrophotometry to determine the concentration of Rhodamine B contained in the samples. The results of the qualitative analysis using the rapid test kit showed that, out of 17 lipstick samples tested, 5 samples were suspected of containing Rhodamine B, namely samples 4, 5, 11, 13, and 15. Quantitative analysis using UV–Vis spectrophotometry revealed that the Rhodamine B concentrations were 0.1213 mg/g in sample 4, 0.0861 mg/g in sample 5, 0.0584 mg/g in sample 11, 0.0961 mg/g in sample 13, and 0.1082 mg/g in sample 15. Based on these findings, it can be concluded that several lipsticks suspected of containing hazardous dyes, such as Rhodamine B, are still found in the market and it urges the stakeholder to be more concerned about the dangerous ingredients in cosmetics.

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Journal Info

Abbrev

jpns

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology

Description

The Journal of Pharmaceuticals and Natural Sciences (JPNS; ISSN 3047-5457) is a scientific, open-access, peer-reviewed journal published by B-CRETA Publisher (CV. Borneo Citra Kreatama). The journal publishes three issues per year) and is available only in the online format. JPNS publishes ...