Peanut (Arachis hypogaea L.) is an important legume crop with high economic value, widely used for food consumption and industrial raw materials. Optimizing cultivation techniques is essential to improve its growth and yield. This study aimed to evaluate the effects of plant spacing and frequency of biofertilizer application on the growth and yield performance of peanut plants. The experiment was conducted using a factorial Completely Randomized Design (CRD) with two factors: plant spacing (30 × 20 cm, 30 × 25 cm, and 30 × 30 cm) and frequency of biofertilizer application (three and four applications). Each treatment combination was replicated three times. Observed parameters included plant height, flowering time, harvesting time, number of filled pods per plant, dry pod weight, number of filled seeds per plant, and seed weight per plant. Data were analyzed using analysis of variance followed by Duncan’s New Multiple Range Test (DNMRT) at the 5% significance level. The results showed that no interaction occurred between plant spacing and biofertilizer application frequency for all observed parameters. However, plant spacing of 30 cm × 25 cm and four applications of biofertilizer consistently produced better growth and yield components compared to other treatments. These findings indicate that appropriate plant spacing combined with an optimal frequency of biofertilizer application can enhance peanut productivity by improving soil microorganism activity and nutrient availability.
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