Indonesian Journal of Health Research and Development
Vol. 4 No. 2 (2026): Indonesian Journal of Health Research and Development

Utilization of Nipa Palm (Nypa fruticans) Leaf Extract as a Natural Preservative for Salted Three Spot Gourami (Trichopodus trichopterus)

Agustin Maharani Khairunisa (University of PGRI Palembang)
Riya Liuhartana Nasyiruddin Fuhrmann (University of PGRI Palembang)
Rih Laksmi Utpalasari (University of PGRI Palembang)
Zhulian Hikmah Hasibuan (University of PGRI Palembang)
RR. Dyah Paramitha Mentari (University of PGRI Palembang)
Steven Suryoprabowo (Bina Nusantara University)
Muhammad Shoaib (Zhejiang University)



Article Info

Publish Date
23 Jun 2026

Abstract

Background: Salted three-spot gourami (Trichopodus trichopterus) is a traditional fish product that is susceptible to microbial spoilage and quality deterioration during storage. The use of plant-based bio-preservatives has been explored as an alternative to synthetic additives to improve product safety and shelf life.  Aims: This study aimed to assess the effectiveness of nipa palm (Nypa fruticans) leaf extract at different concentrations as a natural preservative for salted three-spot gourami.Methods: The experiment was arranged in a Completely Randomized Design (CRD) with four treatments (0%, 10%, 15%, and 20%) and three replications. Parameters observed included moisture content, Total Plate Count (TPC), and organoleptic attributes (appearance, aroma, and texture).Results: The application of nipa palm leaf extract significantly (P < 0.05) affected moisture content, microbial growth, and sensory quality during storage. The 15% treatment (P2) produced the lowest moisture content throughout storage, reaching 19.83% on day 30. Microbial counts in all extract-treated samples remained within the maximum permissible limit of the Indonesian National Standard (≤1 × 10⁵ CFU/g), with the 20% treatment (P3) showing the lowest microbial load (1.0 × 10⁵ CFU/g) on day 30. Sensory evaluation indicated that extract-treated samples maintained acceptable appearance, aroma, and texture throughout storage, with better sensory stability than the untreated control.Conclusion: Nipa palm leaf extract effectively improved the storage quality of salted three-spot gourami. Although the 15% concentration (P2) achieved the greatest moisture reduction, the 20% concentration (P3) is recommended for practical application because it provided the strongest microbial inhibition while maintaining acceptable sensory quality during 30 days of storage.

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Journal Info

Abbrev

ijhrd

Publisher

Subject

Health Professions Medicine & Pharmacology Nursing Public Health

Description

Indonesian Journal of Health Research and Development with E-ISSN: 2987-9671 is a journal managed by CV Media Inti Teknologi. Publish articles on Research activities in the fields of : 1. Nursing 2. Medicine 3. Pharmacy 4. Health Innovation 5. Kebidanan (Bostetrics) 6. Healthy Education 7. Nutrition ...