Journal of Tropical Mycorrhiza
Vol. 5 No. 1 (2026): April

Single-Spore Culture of Racocetra crispa Using a Legume Tree Species

Faisal Danu Tuheteru (Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.)
Deli (Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.)
Husna Husna (Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.)
Asrianti Arif (Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.)
Wiwin Rahmawati Nurdin (Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.)



Article Info

Publish Date
30 Apr 2026

Abstract

The propagation of Racocetra crispa using tree-like legume host plants has not yet been widely undertaken. This study aims to investigate the sporulation of R. crispa in single-spore cultures using legume tree host plants, namely sengon (Paraserianthes falcataria), albizia (Albizia saponaria), johar (Cassia siamea), cajanus (Cajanus cajan), and lamtoro (Leucaena leucocephala). The study was conducted at the Greenhouse of the Indonesian Mycorrhiza Association (AMI) Southeast Sulawesi Branch and the Forestry Department Laboratory of Halu Oleo University from November 2025 to February 2026. The study employed a completely randomised design (CRD) with 5 treatments and 3 replicates. The variables observed included culture success, AMF colonisation of the roots, and the number of spores formed at 3 months, 5 months, and after drying 10 g of medium. The results of the study showed that the A. saponaria host plant treatment yielded the best results, with a culture success rate of 86.67%, the highest AMF colonisation of 35.83%, and the highest number of spores at 5 months and after drying 10g of medium. Conversely, L. leucocephala showed the lowest results, with a culture success rate and spore count of 0%. The best host plant for the propagation of Racocetra crispa is A.saponaria.

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Journal Info

Abbrev

JTM

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Journal of Tropical Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The journal covers research into mycorrhizas, including molecular biology of the plants and fungi, fungal ...