<![CDATA[Green-synthesized bimetallic copper oxide–zinc oxide (CuO/ZnO) nanoparticles have attracted considerable interest due to their enhanced physicochemical properties and antibacterial activity. This study proposes a green synthesis approach for CuO/ZnO nanoparticles and evaluates their physicochemical characteristics and antibacterial performance by means of a range of analytical techniques including XRD, XRF, FTIR, SEM–EDX, and UV–Visible spectroscopy. XRD analysis confirmed elevated crystallinity in all samples, while the CuO/ZnO bimetallic variant (Cbest) exhibited the smallest crystallite size (22.93 nm). Antibacterial assays, conducted utilizing the agar well diffusion method demonstrated that pure CuO nanoparticles exhibited the strongest inhibition against Escherichia coli (15.39 mm), whereas the CuO/ZnO bimetallic variant (Cbest) demonstrated the highest activity against Staphylococcus aureus (10.02 mm). The results of the one-way ANOVA indicated significant differences among the treatments (p < 0.05), thus confirming the influence of nanoparticle composition on antibacterial efficacy. These findings highlight the potential of green-synthesized CuO/ZnO nanoparticles as effective antibacterial agents.]]>
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