I Wayan Karyasa
Department of Chemistry; Universitas Pendidikan Ganesha, Singaraja 81117, Indonesia

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Physicochemical and antibacterial properties of green-synthesized bimetallic CuO-ZnO nanoparticles Ni Ketut Eri Suryani; I Wayan Karyasa; I Putu Parwata
Communications in Science and Technology Vol 11 No 1 (2026)
Publisher : Komunitas Ilmuwan dan Profesional Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21924/cst.11.1.2026.1976

Abstract

Green-synthesized bimetallic copper oxide–zinc oxide (CuO/ZnO) nanoparticles have attracted considerable interest due to their enhanced physicochemical properties and antibacterial activity. This study proposes a green synthesis approach for CuO/ZnO nanoparticles and evaluates their physicochemical characteristics and antibacterial performance by means of a range of analytical techniques including XRD, XRF, FTIR, SEM–EDX, and UV–Visible spectroscopy. XRD analysis confirmed elevated crystallinity in all samples, while the CuO/ZnO bimetallic variant (Cbest) exhibited the smallest crystallite size (22.93 nm). Antibacterial assays, conducted utilizing the agar well diffusion method demonstrated that pure CuO nanoparticles exhibited the strongest inhibition against Escherichia coli (15.39 mm), whereas the CuO/ZnO bimetallic variant (Cbest) demonstrated the highest activity against Staphylococcus aureus (10.02 mm). The results of the one-way ANOVA indicated significant differences among the treatments (p < 0.05), thus confirming the influence of nanoparticle composition on antibacterial efficacy. These findings highlight the potential of green-synthesized CuO/ZnO nanoparticles as effective antibacterial agents.