The purpose of this study was to determine the effect of organizational culture and information technology on employees’ performance through job satisfaction in PT SUCOFINDO SBU Laboratorium. The data collection method used a questionnaire. The data analysis methods used in this study were path analysis and soble test. The results of this study indicate that organizational culture has a positive and significant effect on job satisfaction, information technology has a positive and significant effect on job satisfaction, organizational culture has a positive and significant effect on employee performance, information technology has a positive and significant effect on employee performance, job satisfaction has a positive and no significant effect on employee performance, organizational culture has a positive and no significant effect on employee performance through job satisfaction, and information technology has a positive and significant effect on employee performance through job satisfaction. This study concludes that information technology plays a crucial role in enhancing employee performance, both directly and through job satisfaction. The theoretical implication of this research is the confirmation of job satisfaction's mediating role in the relationship between information technology and employee performance. Practically, the findings suggest that PT SUCOFINDO SBU Laboratorium should focus on improving organizational culture, optimizing information technology utilization, and enhancing job satisfaction to boost employee performance. Future research is recommended to explore additional variables and expand the research context to different industries.
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