AL KAUNIYAH
Vol. 19 No. 2 (2026): AL-KAUNIYAH: JURNAL BIOLOGI

The Effect of NaFeEDTA as an Iron Fortificant an Hematocrit and Blood Platelet Levels of Rats (Rattus norvegicus L.) Sprague-dawley Strain

Nova Anita (Universitas Indonesia)
Agustino Zulys (Universitas Indonesia)
Amelia Rosvita (Universitas Indonesia)
Aisyah Safrina (Universitas Indonesia)



Article Info

Publish Date
09 Jul 2026

Abstract

The potential of NaFeEDTA as an iron fortificant can be assessed by identifying hemoglobin and red blood cell levels and their relationship to hematocrit levels. High hematocrit values can affect blood circulation. Iron can also affect platelet levels, excessive platelet aggregation can inhibit blood flow.  This study aims to identify hematocrit and blood platelet levels of rats after NaFeEDTA in soy milk (SM). The method is RAL with 25 male rats in 5 treatment groups: KK1 (without SM); KK2 (SM 10 mL/kgBW); KP1 (SM + NaFeEDTA 2.7 mg Fe/kgBW); KP2 (SM + NaFeEDTA 5.4 mg Fe/kgBW); KP3 (SM + NaFeEDTA 10.8 mg Fe/kgBW) for 14 days. Hematocrit was measured using a hematology analyzer (t0 and t14). ANOVA-LSD test (P <0.05) showed hematocrit increases in KK2, KP1, KP2, and KP3 compared to KK1, highest in KP2 (6.22%). NaFeEDTA significantly reduced platelet levels, highest decrease in KP2 (3.06% compared to KK1 and 2.81% compared to KK2). Despite the decrease, platelet levels remained normal. Thus, NaFeEDTA has the potential to fortify iron absorption at an optimal dose of 5.4 mg Fe/kgBW.

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Journal Info

Abbrev

kauniyah

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology

Description

Al-Kauniyah: Jurnal Biologi (p-ISSN: 1978-3736, e-ISSN: 2502-6720) is an Open Access Journal published by Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, and established since 2007. Since 2016 Al-Kauniyah has established a collaboration ...