IN VITRO ANTIBACTERIAL EFFECT OF DIODE LASER ON Porphyromonas gingivalis ASSOCIATED WITH PERIODONTITIS

Dwi Wahyu Indrawati (Department of Dentistry, Faculty of Dentistry, Universitas Muhammadiyah Sidoarjo, Sidoarjo,)
Anis Khoirin Hayati (Department of Dentistry, Faculty of Dentistry, Universitas Muhammadiyah Sidoarjo, Sidoarjo,)
Rizky Briliant Syah Manurung (Resident of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya)
Muhammad Rizky Akbar Manurung (Undergraduate Student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya,)
Agus Hayatal Falah (Department of Electrical Engineering, Faculty of Science and Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo,)

Journal Of Vocational Health Studies
Vol. 10 No. 1 (2026): July 2026 | JOURNAL OF VOCATIONAL HEALTH STUDIES

Background: Antibiotic resistance has become a major global health concern, including in periodontal therapy. Therefore, alternative antimicrobial approaches that do not rely on antibiotics are required. Purpose: This study aims to evaluate the in vitro antibacterial effect of the diode laser on Porphyromonas gingivalis associated with periodontitis. Method: This study employed an in vitro experimental design with a control group and a treatment group. A diode laser with a wavelength of 660 - 900 nm was applied to the P. gingivalis cultures with exposure times of 5, 10, and 15 seconds. The bacterial growth was assessed by measuring the inhibition zone diameter. Result: The results demonstrated a significant reduction in P. gingivalis growth following diode laser irradiation, with longer exposure times producing larger inhibition zones. Conclusion: Diode laser irradiation exhibited significant in vitro antibacterial activity against P. gingivalis. These findings indicate that diode lasers have potential as an alternative antimicrobial modality in periodontal therapy and warrant further investigation through in vivo and clinical studies.