Cellulolytic and hemicellulolytic bacteria from the gut of Oryctes rhinoceros larvae

SITI LUSI ARUM SARI (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
ARTINI PANGASTUTI (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
ARI SUSILOWATI (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
TJAHJADI PURWOKO (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
EDWI MAHAJOENO (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
WAHYU HIDAYAT (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
IKOW MARDHENA (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
DEWI KURNIAWATI (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
ROBIAH ANITASARI (Departement of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)

Biodiversitas Journal of Biological Diversity
Vol. 17 No. 1 (2016)

Sari SLA, Pangastuti A, Susilowati A, Purwoko Tj, Mahajoeno E, Hidayat W, Mardhena I, Panuntun DF, Kurniawati D, Anitasari R. 2016. Cellulolytic and hemicellulolytic bacteria from the gut of Oryctes rhinoceros larvae. Biodiversitas 17: 78-83. Lignocellulose is very potential as raw material for biofuel production because it is cheap, abundant and renewable. The main carbohydrate constituents of lignocellulosic material are cellulose and hemicelluloses rd instars were used in this research. The research succeeded to isolate 11 bacterial isolates from the gut of O. rhinoceros larvae. The screening result demonstrated that bacterial isolates had cellulolytic (72.7% of total isolates), and mannanolytic (100% of total isolates) activity. Based on the 16S rDNA sequence, 10 isolates were classified into Bacillus and only 1 isolate was classified into Citrobacter. The GOR2 which was closely related to Bacillus pumilus vit bac1 has the highest cellulolytic and xylanolytic activities. The isolate with the highest mannanolytic activity was the GOR7 which was closely related to Bacillus aryabhattai strain IHB B 6821.