Biodiversitas Journal of Biological Diversity
Vol. 11 No. 3 (2010)

Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers

YUYU SURYASARI POERBA (Botany Division, Research Centre for Biology, The Indonesia Institute for Sciences (LIPI))
FAJARUDIN AHMAD (Botany Division, Research Centre for Biology, The Indonesia Institute for Sciences (LIPI))



Article Info

Publish Date
31 May 2010

Abstract

Poerba YS, Ahmad F (2010) Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers. Biodiversitas 11: 118-123. This study was done to assess the molecular diversity of 36 accessions (18 cultivars) of the plantain and cooking bananas (Musa acuminata x M. balbisiana, AAB, ABB subgroups) based on Random amplified polymorphic DNA (RAPD) and and Inter Simple Sequence Repeats (ISSR) markers and to determine genetic relationships in the bananas. RAPD and ISSR fingerprinting of these banana varieties was carried out by five primers of RAPDs and two primers of ISSRs. RAPD primers produced 63 amplified fragments varying from 250 to 2500 bp in size. 96.82% of the amplification bands were polymorphic. ISSR primers produced 26 amplified fragments varying from 350 bp to 2000 bp in size. The results showed that 92.86% of the amplification bandswere polymorphic. The range of genetic distance of 18 cultivars was from 0.06-0.67.Key words: RAPD analysis, Musa acuminata, Musa balbisiana, plantain, cooking bananas.

Copyrights © 2010






Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...