Biodiversitas Journal of Biological Diversity
Vol. 10 No. 4 (2009)

Genetic Relationship of Sago Palm (Metroxylon sagu Rottb.) in Indonesia Based on RAPD Markers

BARAHIMA ABBAS (Faculty of Agriculture and Technology, State University of Papua (UNIPA), Manokwari 98314, Indonesia)
MUHAMMAD HASIM BINTORO (Faculty of Agriculture, Bogor Agricultural University (IPB), Bogor 16680, Indonesia)
SUDARSONO SUDARSONO (Faculty of Agriculture, Bogor Agricultural University (IPB), Bogor 16680, Indonesia)
MEMEN SURAHMAN (Faculty of Agriculture, Bogor Agricultural University (IPB), Bogor 16680, Indonesia)
HIROSHI EHARA (Faculty of Bioresources, Mie University, 1577 Kurimamachiya,Tsu-city, Mie-Pref. 514-8507)



Article Info

Publish Date
20 Jul 2009

Abstract

The areas of sago palm (Metroxylon sagu Rottb.) forest and cultivation in the world were estimated two million hectares andpredicted 50% of that areas located in Indonesia. Distribution of sago palm areas in Indonesia is not evenly distributed as well as their diversities. Information of plant genetic diversities and genetic relationship is very important to be used for germplasm collection and conservation. The objectives of research were revealed the genetic relationships of sago palm in Indonesia based on RAPD molecular markers. Fragments amplification PCR products were separated on 1.7% agarose gel, fixation in Ethidium Bromide, and visualized by using Densitograph. Genetic relationships of sago palm in Indonesia showed that sample in individual level were inclined mixed among the other and just formed three groups. Genetic relationship of sago palm population showed that samples populations from Jayapura, Serui, Sorong, Pontianak, and Selat Panjang were closely related each others based on phylogenetic analysis and formed clustered in one group, event though inclined to be formed two subgroups. Populations from Manokwari, Bogor, Ambon and Palopo were closed related each others, they were in one group. Genetic relationships in the level of island were showed sago palm from Papua, Kalimantan, and Sumatra closely related. Sago palms from Maluku were closed related with sago palm from Sulawesi whereas sago palm from Java separated from the others. Based on this observation we proposed that Papua as centre of sago palm diversities and the origin of sago palm in Indonesia. This research informed us the best way to decide sago palm places for germplasm of sago palm conservation activity.Key words: genetic relationships, population, sago palm, RAPD, Indonesia

Copyrights © 2009






Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...