Biodiversitas Journal of Biological Diversity
Vol. 9 No. 3 (2008)

Morphological and isozymic banding pattern study of white grubs (Coleoptera: Melolonthidae) as pest of bark crop in mounth Merapi’s slope.

SUGIYARTO SUGIYARTO (Jurusan Biologi FMIPA, Universitas Sebelas Maret (UNS), Surakarta 57126. Program Studi Biosains Program Pascasarjana, Universitas Sebelas Maret (UNS), Surakarta 57126. Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas LPPM, Universit)



Article Info

Publish Date
28 May 2008

Abstract

White grub (Coleoptera: Melolonthidae) is a group of soil pest at any agrosystem., especially at Salak pondoh (Salacca zalacca (Gaert.)Voss.) crop. The characteristics of this specimen were very crucial to be studied in order to find the exact biocontrol. The aim of this research was to know the characteristics of white grubs (Melolonthidae: Coleoptera) based on morphological and isozyme banding patterns. This research was conducted on August - November 2007 at Sleman and Magelang districts for the morphological purposes, while for the isozyme data were conducted at Sub Laboratory Biology, Central Laboratory of Sebelas Maret University Surakarta. Sample was taken by using stratified random sampling method, on five stations. Polyacrylamide gel electrophoresis (PAGE) using the vertical type was taken to isozyme analysis. The enzyme used in this research were peroxidase and esterase to detect the isozyme banding patterns. The results showed that there was no morphological variation of white grubs (Melolonthidae: Coleoptera) at salak pondoh agroecosystem in Mounth Merapi’s slope. Based on this character, there was one species of white grub found, i.e. Holotrichia javana. There was a genetic variation based on the variation of isozyme banding patterns.© 2008 Jurusan Biologi FMIPA UNS Surakarta.Key words: white grubs, isozyme, Merapi mount, bark.

Copyrights © 2008






Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...