Biodiversitas Journal of Biological Diversity
Vol. 8 No. 1 (2007)

Genetic uniformity of sungkai (Peronema canescens Jack) regenerated from tissue culture

MARIA IMELDA (Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Cibinong-Bogor 16911)
AMY ESTIATI (Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Cibinong-Bogor 16911)
LAELA SARI (Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Cibinong-Bogor 16911)
FEBRYANA ERLYANDARI (Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Cibinong-Bogor 16911)



Article Info

Publish Date
01 Jan 2007

Abstract

Sungkai or jati sebrang (Peronema canescens Jack) is one of the industrial timber estate species native to Indonesia, which is commonly chosen for reforestation and as raw materials for the furniture and handicraft industry. In order to provide this planting material in large and sustainable quantities, a technique for in vitro propagation of sungkai through adventitious shoot proliferation is needed and has been successfully developed at the Research Centre for Biotechnology, LIPI. Since tissue culture method is prone to genetic variations, it is important to assess the genetic uniformity of sungkai planting materials derived from this in vitro method at an early stage. In this research, early detection of genetic uniformity was done by morphological observation of the regenerant plants and RAPD analysis using 4 primers namely OPB 5, OPB 9, OPH 11 and OPH 19. Morphological test showed differences in leaf shape, stem diameter and plant height among plantlets originating from Kalbar, Kaltim, Jambi and Cibinong. However, RAPD analysis with PCR showed that all planting materials were genetically uniform among those originating from the same or different places.© 2007 Jurusan Biologi FMIPA UNS SurakartaKey words: sungkai (Peronema canescens Jack), perbanyakan in vitro, morfologi, RAPD

Copyrights © 2007






Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...