Biodiversitas Journal of Biological Diversity
Vol. 6 No. 4 (2005)

Phylogenetic analyses of rhizobia isolated from Aeschynomene spp.

EVI TRIANA (Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Bogor 16002)



Article Info

Publish Date
01 Jan 2005

Abstract

The 16S rDNA sequence of eleven strains isolated from nodules of Aeschynomene spp. have been determined and analyses together with sequence of other related taxa. Four bacterial groups were identified: (i) photosynthetic Bradyrhizobial group consist of IRBG2, IRBG228 dan IRBG 230; (ii) non-photosintetik Bradyrhizobium group consist of MAFF210127, MAFF210316, MAFF210318, dan MAFF210408; (iii) Rhodopseudomonas palustris group consist of HMD88, HMD89 dan 99D and (4) an isolate, 99C, that is related to Rhodopseudomonas acidophila. In addition, DNA-DNA hybridization was performed among HMD88, HMD89 and 99D strains. The result showed that DNA homology of HMD88, HMD89 and 99D with Rps. palustris were less than 30%, DNA homology of HMD88, HMD89 and Rps. Palustris with 99D is about or less than 20%, otherwise between HMD88 and HMD89, DNA similarity was more than 70%. The result suggest that HMD88 and HMD89 are the same species, meanwhile 99D is close related or different species in Rhodopseudomonas palustris group. Key words: 16S rDNA sequence, Aeshynomene sp., photosynthetic rhizobia, DNA-DNA hybridization, Rhodopseudomonas palustris

Copyrights © 2005






Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...