Identification and characterisation of the polymorphic growth hormone gene of the Bali cattle, the Madura cattle and Ongole cattle

SUTARNO SUTARNO (Jurusan Biologi FMIPA UNS Surakarta 57126)
ARIS JUNAIDI (Fakultas Kedokteran Hewan UGM Yogyakarta 55281)
AGUS PURWOKO (Jurusan Biologi FMIPA UNS Surakarta 57126)
NEO INDRA LELANA (Jurusan Biologi FMIPA UNS Surakarta 57126)

Biodiversitas Journal of Biological Diversity
Vol. 3 No. 1 (2002)

A total of 150 cattle consisting of 3 breeds (Bali, Madura and Ongole) of Indonesian native cattle were used in this study. The aims of the study were to identify and characterize polymorphisms in the growth hormone loci of those breeds of cattle. The genomic DNA was extracted using Wizard genomic DNA purification system from Promega. Two fragments of growth hormone gene were amplified using PCR and continued with RFLP using restriction enzymes of AluI and MspI. Polymorphisms were found at both loci of GH-L1 and GH-L2 of the growth hormone gene by PCRRFLP analysis in all breeds of those cattle.© 2002 Jurusan Biologi FMIPA UNS SurakartaKeywords: PCR-RFLP, growth hormone gene, polymorphism, Indonesian local cattle.