Biodiversitas Journal of Biological Diversity
Vol. 18 No. 2 (2017)

Short Communication: Cytological studies on black orchid hybrid (Coelogyne pandurata Lindley)

SRI HARTATI (Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia)
NANDARIYAH NANDARIYAH (Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia)
AHMAD YUNUS (Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia)
DJATI W. DJOAR (Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia)



Article Info

Publish Date
13 Feb 2017

Abstract

Hartati S, Nandariyah, Yunus A, Djoar DW. 2017. Cytological studies on black orchid hybrid (Coelogyne pandurata Lindley). Biodiversitas 18: 555-559. Black orchid (Coelogyne pandurata Lindley) is one of the rare species of Coelogyne Lindley, protected by the government of Indonesia. The cytological character of orchid is very important to study to support the success of breeding. The study aimed to assess the chromosomal number, karyotype pattern and ploidy level the hybrid of Black Orchid (Coelogyne pandurata). This study shows that the chromosomal number of the hybrid of Coelogyne pandurata (2n = 36) >< Coelogyne rumphii (2n = 72) is 2n = 54. Ploidy analysis by flow cytometry shows that hybrid shows triploid (2n = 3x = 54) different from the parent Coelogyne pandurata which is diploid (2n = 2x = 36) and the parent Coelogyne rumphii which is tetraploid (2n = 4x = 72). However, both parents and their hybrid performed the same karyotype pattern, which is metacentric. The chromosome size showed a variation in length from the longest Coelogyne pandurata 2.98 ± 0.15 µm to the shortest Coelogyne rumphii 2.24 ± 0.15 µm. The hybrids have a range of 2.50 ± 0,10 µm to 2.85 ± 0.10 µm. Karyotype patterns of black orchid (C. pandurata), C. rumphii and the hybridis metacentric.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...