Biodiversitas Journal of Biological Diversity
Vol. 18 No. 4 (2017)

Use of endophytic bacteria from roots of Cyperus rotundus for biocontrol of Meloidogyne incognita

MARDHIANA MARDHIANA (Program of Agrotechnology, Faculty of Agriculture, Universitas Borneo Tarakan. Jl Amal Lama No 1, Tarakan 77123, North Kalimantan, Indonesia)
ANKARDIANSYAH PANDU PRADANA (Program of Agrotechnology, Faculty of Agriculture, Universitas Borneo Tarakan. Jl Amal Lama No 1, Tarakan 77123, North Kalimantan, Indonesia)
MUH ADIWENA (Program of Agrotechnology, Faculty of Agriculture, Universitas Borneo Tarakan. Jl Amal Lama No 1, Tarakan 77123, North Kalimantan, Indonesia)
DWI SANTOSO (Program of Agrotechnology, Faculty of Agriculture, Universitas Borneo Tarakan. Jl Amal Lama No 1, Tarakan 77123, North Kalimantan, Indonesia)
RIZZA WIJAYA (Program of Agrotechnology, Faculty of Agriculture, Universitas Borneo Tarakan. Jl Amal Lama No 1, Tarakan 77123, North Kalimantan, Indonesia)
ADITYA MURTILAKSONO (Program of Agrotechnology, Faculty of Agriculture, Universitas Borneo Tarakan. Jl Amal Lama No 1, Tarakan 77123, North Kalimantan, Indonesia)



Article Info

Publish Date
04 Oct 2017

Abstract

Mardhiana, Pradana AP, Adiwena M, Santoso D, Wijaya R, Murtilaksono A. 2017. Use of endophytic bacteria from roots of Cyperus rotundus for biocontrol of Meloidogyne incognita. Biodiversitas 18: 1308-1315. Yield loss due to M. incognita infection in tomato plants cultivation can reach 60%. The problem is able to be solved through the application of endophytic bacteria. In this study, endophytic bacteria from root Cyperus rotundus were isolated using Tryptic Soy Agar media. The bacteria isolates were then tested their safety against plants and mammals. The phenotypic and physiological properties of selected isolates were characterized and tested to know their resistance to antibiotics, and their ability in suppressing the infection rate of M. incognita on tomato. Eighteen bacterial isolates were obtained and 8 of them are categorized as safe bacteria for plants and mammals, which could be used in further tests. A result of the physiological test showed that bacterial isolates were able to produce protease enzyme (87.5%), chitinase enzyme (62.5%), and HCN (37.5%), having urease activity (75%) and could dissolve phosphate (87.5%). Based on the test results, all endophytic bacteria effectively increased tomato growth and suppressed the severity of M. incognita infection with the most stable isolate as a biocontrol agent of M. incognita was CRS16.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...