Biodiversitas Journal of Biological Diversity
Vol. 20 No. 3 (2019)

Short Communication: Molecular characteristic of taro white cattle based on DNA microsatellite markers

LUH GEDE SRI SURYA HERYANI (Department of Anatomy, Faculty of Veterinary Medicine, Universitas Udayana. Jl. P.B. Sudirman, Denpasar 80234, Bali, Indonesia)
I NENGAH WANDIA (Department of Anatomy, Faculty of Veterinary Medicine, Universitas Udayana. Jl. P.B. Sudirman, Denpasar 80234, Bali, Indonesia)
I WAYAN SUARNA (Department of Tropical Forage Research and Development Center, Universitas Udayana. Jl. P.B. Sudirman, Denpasar 80234, Bali, Indonesia)
I KETUT PUJA (Department of Veterinary Genetics and Reproduction Technology, Faculty of Veterinary Medicine, Universitas Udayana, Jl. P.B. Sudirman, Denpasar 80234, Bali, Indonesia)
NI NYOMAN WERDI SUSARI (Department of Anatomy, Faculty of Veterinary Medicine, Universitas Udayana. Jl. P.B. Sudirman, Denpasar 80234, Bali, Indonesia)
KADEK KARANG AGUSTINA (Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Udayana. Jl. P.B. Sudirman, Denpasar 80234, Bali, Indonesia)



Article Info

Publish Date
14 Feb 2019

Abstract

Abstract. Heryani LGSS, Wandia IN, Suarna IW, Puja IK, Susari NNW, Agustina KK. 2019. Short Communication: Molecular characteristic of taro white cattle based on DNA microsatellite markers. Biodiversitas 20: 671-675. This research was conducted to assess and characterize the genetics of Taro White cattle. Genetic characterizations of this cattle are essential to conservation and breeding program. A total of 18 samples and 4 pairs of microsatellite DNA markers (BM2113, BM1824, INRA023, and ETH225) were amplified by PCR and the products were run on 8% bis-Acrylamide gels. All microsatellite markers were successfully amplified with a mean allelic number of 3.25. Means of observed and expected heterozygosity were found to be 0,25 and 0,628. The Polymorphism Information Content (PIC) values ranged from 0.448 (BM1824) to 0.627 (BM2113) and fixation index were 0.620. The deviation from Hardy-Weinberg equilibrium revealed that Taro White cattle population exhibited significant deviations from Hardy-Weinberg equilibrium (HWE) and possessed a possibility of inbreeding. The microsatellite loci used or focused in the present study further validate their use for evaluation of genetic diversity of Taro White

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...