Biodiversitas Journal of Biological Diversity
Vol. 19 No. 3 (2018)

Identification and characterization of Edwardsiella ictaluri from diseased Pangasius pangasius, cultured in Cirata Lake, Indonesia

MIRA MAWARDI (Main Center for Freshwater Aquaculture Development, Ministry of Marine Affair and Fisheries, Indonesia. Jl. Selabintana No. 37, Sukabumi 43151, West Java, Indonesia.)
JAELANI JAELANI (Main Center for Freshwater Aquaculture Development, Ministry of Marine Affair and Fisheries, Indonesia. Jl. Selabintana No. 37, Sukabumi 43151, West Java, Indonesia.)
ZAKKI ZAINUN (Main Center for Freshwater Aquaculture Development, Ministry of Marine Affair and Fisheries, Indonesia. Jl. Selabintana No. 37, Sukabumi 43151, West Java, Indonesia.)
YUANI MUNDAYANA (Main Center for Freshwater Aquaculture Development, Ministry of Marine Affair and Fisheries, Indonesia. Jl. Selabintana No. 37, Sukabumi 43151, West Java, Indonesia.)
BAIRD SAM CHILORA (National Aquaculture Research Center, Department of Fisheries, Malawi. P.O Box 229, Domasi, Zomba, Malawi.)
ESTI HANDAYANI HARDI (Faculty of Fisheries and Marine Sciences, Universitas Mulawarman. Jl. Gunung Tabur, Kampus Gunung Kelua, Samarinda 75124, East Kalimantan, Indonesia.)



Article Info

Publish Date
01 May 2018

Abstract

Mawardi M, Jaelani, Zainun Z, Mundayana Y, Chilora BS, Hardi EH. 2018. Identification and characterization of Edwardsiella ictaluri from diseased Pangasius pangasius, cultured in Cirata Lake, Indonesia. Biodiversitas 19: 766-772. In January 2016, there were reported extensive mortalities of Pangasius pangasius cultured on cage, in Cirata Lake. Edwardsiella ictaluri is primarily recognized as a disease-causing pathogen in catfish species, causing an economically important bacterial infection hence affecting productivity of aquaculture enterprises in many regions across the E. ictaluri. The samples were 17 fishes collected from organs containing spleen, kidney, and liver. The total samples were 51 organs of fishes. The high bacteria infection prevalence level was found in kidney (29.41%). The gross pathological signs of sample of organs were abnormal sizes and abnormal color including white nodules. Bacteria were identified by conventional biochemical test, polymerase chain reaction (PCR) analysis and nucleotide sequencing. The results showed that P. pangasius cultured in cages in Cirata Lake is positively infected by E. ictaluri strain (accession number KF9071291). Other infection cases by Aeromonas sp. and Plesiomonas shigelloides as co-infection bacteria in P. pangasius were also found.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...