Biodiversitas Journal of Biological Diversity
Vol. 19 No. 5 (2018)

Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats

SUZAN AHMED BIN-SALMAN (Department of Biology, Faculty of Science, King Abdulaziz University. Al Ehtifalat St, Jeddah 21589, Saudi Arabia)
REDA HASSAN AMASHA (Department of Biology, Faculty of Science, King Abdulaziz University. Al Ehtifalat St, Jeddah 21589, Saudi Arabia.)
SAMYAH D. JASTANIAH (Department of Biology, Faculty of Science, King Abdulaziz University. Al Ehtifalat St, Jeddah 21589, Saudi Arabia.)
MAGEDA M. ALY (Department of Biology, Faculty of Science, King Abdulaziz University. Al Ehtifalat St, Jeddah 21589, Saudi Arabia.)
KHALIL ALTAIF (Faculty of Pharmacy, Middle East University. Queen Alia Airport Street, Amman, Jordan)



Article Info

Publish Date
21 Sep 2018

Abstract

Bin-Salman SA, Amasha RH, Jastaniah SD, Aly MM, Altaif K. 2018. Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats. Biodiversitas 19: 1828-1834. Saline habitats, like the Dead Sea, are unusual extreme environments, due to their extreme salinity. Most saline habitats originate from the evaporation of seawater, and have a nearly neutral to slightly alkaline pH (such as the Red Sea (pH8.3) and Arabian Gulf, pH8.3). Ten halophilic bacterial strains (two Gram-negative) belonging to the family of Halomonadaceae and (eight Gram-positive), belonging to the family of Bacillaceae, were isolated from the Red Sea, Arabian Gulf, and Dead Sea by subjecting the isolates to a high salinity medium, followed by identification using 16S rRNA gene sequencing. Four of isolates were designated on the basis of their tolerance to high salinity; SBR1 exhibited 97% homology to Halomonas aquamarina, SBR2 showed 97% homology to Sediminibacillus sp., (Red Sea), SBA9 exhibited 94% homology to Halobacillus sp., (Arabian Gulf) and SBD17 gave 98% homology to Halobacillus dabanensis (Dead Sea). The isolates were also characterized by their physiological parameters, SBR1 showed optimum growth at 30°C, pH8.5 and1.5M NaCl, SBR2 at 30°C, pH6.0 and 1M NaCl. Optimum conditions for SBA9 were 35°C, pH6.5 and 1M NaCl and for SBD17, 37°C, pH7.0 and 1M NaCl.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...