Biodiversitas Journal of Biological Diversity
Vol. 19 No. 2 (2018)

Molecular characterization of rhizobacteria isolated from walnut (Juglans regia) rhizosphere in Western Himalayas and assessment of their plant growth promoting activities

GH. HASSAN DAR (Division of Environmental Sciences, S.K. University of Agricultural Sciences & Technology. Shalimar, Srinagar 190 025, Jammu & Kashmir, India)
SHAKEELA SOFI (Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology, Shalimar, Srinagar 190 025, Jammu & Kashmir, India)
S.A. PADDER (Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology, Shalimar, Srinagar 190 025, Jammu & Kashmir, India)
AISHA KABLI (Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology, Shalimar, Srinagar 190 025, Jammu & Kashmir, India)



Article Info

Publish Date
01 Mar 2018

Abstract

Dar GH, Sofi S, Padder SA, Aisha Kabli A. 2018. Molecular characterization of rhizobacteria isolated from walnut (Juglans regia) rhizosphere in Western Himalayas and assessment of their plant growth promoting activities. Biodiversitas 19: 712-719. The present study was aimed to isolate and characterize effective bacteria from the rhizosphere of walnut (Juglans regia) grown in North Western Himalayas and assess their growth promoting potential so that they may, in future, be exploited as biofertilizers. Based on preliminary screening of 98 bacterial isolates obtained from four walnut growing districts of Kashmir valley during survey in 2015, 12 isolates were characterized morpho-biochemically and molecularly basis. On the basis of 16S rDNA sequencing they were identified as Bacillus licheniformis WI 90, B. tequilensis WI 62, B. cereus WI 36, B. subtilis strains WI 63 and WI 65, Micrococcus luteus strains WI 12, WI 41 and WI 80; M. yunnanensis strains WI 60 and WI 30 and Micrococcus sp. strains WI 11 and WI 91. The assessment of these rhizobacteria for plant growth promoting attributes revealed that B. licheniformis WI 90 possessed higher phosphorus solubilization activity (312 mg/L), followed by Micrococcus sp. WI 91 (267 mg/L) while high siderophore was produced by M. luteus WI 12 (27.2% siderophore units), followed by B. licheniformis WI 90. B. cereus strains WI 36. High IAA contents (30 µg/mL) was yielded by WI 41, followed by M. yunnanensis WI 60 (28 µg IAA/mL) while higher and statistically at par gibberellic acid was produced by B. licheniformis WI 90, Micrococcus sp. WI 91 and M. luteus WI 80. Higher chitinase enzyme activity was observed in B. subtilis WI 63 (30.5 units/mL), followed by B. tequilensis WI 62 (25.3 units/mL) and B. subtilis WI 65 (25.1 units/mL). The study revealed high plant growth promoting potential in these rhizobacteria.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...