Biodiversitas Journal of Biological Diversity
Vol. 20 No. 1 (2019)

Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker

SARA SIAHPOUSH (Department of Plant Protection, Lorestan University, P.O. Box 465, Khorramabad, Iran.)
MOSTAFA DARVISHNIA (Department of Plant Protection, Lorestan University, P.O. Box 465, Khorramabad, Iran.)



Article Info

Publish Date
01 Jan 2019

Abstract

Siahpoush S, Darvishnia M. 2019. Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker. Biodiversitas 20: 292-296. Fusarium species cause important disease on many crops including cereals, and accurate identification and then proper management of this disease will be helpful to reduce economic losses. In this paper, 13 Fusarium isolates from root and crown of cereals in western Iran were identified by morphological and molecular methods. Sequencing of translation elongation factor 1-alpha revealed 8 species as follows: Fusarium acuminatum, F. avenaceum, F. culmorum, F. equiseti, F. proliferatum, F. reticulatum, F. solani, and F. tricinctum. Species diversity was analyzed by random amplified polymorphic DNA (RAPD). Four primers were used as Rfu9, Rfu10, Rfu23, and Rfu25 which all of them produced distinct and reproducible bands. A dendrogram was developed by UPGMA. Generated polymorphic bands were observed in all 13 different species by 4 primers. Rfu9 by 13 bands and Rfu23 by 10 ones, produced the most and the least bands respectively. Genetic similarity coefficient was between 0.00-0.9. RAPD analysis showed that these isolates were genetically varied and two clusters were formed with Fusarium isolates.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...