Response of polyisoprenoid concentration and profile in three groups of mangrove seedlings of coping with long-term salinity

MOHAMMAD BASYUNI (Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatera, 20155, Indonesia.)
HIROSHI SAGAMI (Institute of Multidisciplinary Research for Advanced Material, Tohoku University. 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan)
SHIGEYUKI BABA (International Society for Mangrove Ecosystems, Faculty of Agriculture, University of the Ryukyus. 1 Senbaru, Nishihara, Okinawa 903-0213, Japan)
HIROSUKE OKU (Molecular Biotechnology Group, Tropical Biosphere Research Center, University of the Ryukyus. 1 Senbaru, Nishihara Okinawa 903-0213, Japan)

Biodiversitas Journal of Biological Diversity
Vol. 20 No. 1 (2019)

Basyuni M, Sagami H, Baba S, Oku H. 2019. Response of polyisoprenoid concentration and profile in three groups of mangrove seedlings of coping with long-term salinity. Biodiversitas 20: 320-326. The response of polyisoprenoid (polyprenol and dolichol) concentration and distribution was investigated with three groups mechanism of coping with salinity: a secreting mangrove species of Avicennia officinalis, a non-secreting species (excluder) of Bruguiera cylindrica, and a salt-accumulating species of Xylocarpus granatum. The seedlings of three mangroves were grown under 0 and 3% salinity concentration for five months. Polyisoprenoids in the lipid extracts were examined by two-dimensional thin layer chromatography (2D-TLC). The pattern of the polyprenols and dolichols in the leaves and roots were categorized as two types (I and II). In category I, dolichols dominated over polyprenols, however, in category II, the existence of both polyprenols and dolichols was found. In the leaves, type-I was observed in A. officinalis under 0 and 3% salinity. On the other hand, type-II was found in B. cylindrica and X. granatum under 0 and 3% salt concentrations. A similar pattern was found in the roots, A. officinalis (type-I), and that in B. cylindrica and X. granatum was type-II. This finding depicted that the seedlings of A. officinalis, B. cylindrica, and X. granatum leaves and roots imply no change in the distribution type: the categories were distributed as type I or II under 0% salt concentrations, as well as type-I or II under 3% salt concentrations. This study implied that polyisoprenoids may play a protective function against salinity in the mangrove leaves and roots of three groups scheme (secreting, excluding, and accumulating) of salt management.