Biodiversitas Journal of Biological Diversity
Vol. 20 No. 5 (2019)

Phylogenetic study of endophytic fungi associated with Centella asiatica from Bengkulu and Malaysian accessions based on the ITS rDNA sequence

NANI RADIASTUTI (Department of Biology, Faculty of Science and Technology, UIN Syarif Hidayatullah Jakarta. Jl. Ir. H. Djuanda no: 90, Ciputat, Banten. Indonesia)
DWI NINGSIH SUSILOWATI (Indonesian Center for Agricultural Biotechnology and Genetic Resouces Research and Development, West Java, Indonesia)
HUSHSHILA A BAHALWAN (Department of Biology, Faculty of Science and Technology, UIN Syarif Hidayatullah Jakarta. Jl. Ir. H. Djuanda no: 90, Ciputat, Banten. Indonesia)



Article Info

Publish Date
11 Apr 2019

Abstract

Abstract. Radiastuti N, Bahalwan HA, Susilowati DN. 2019. Phylogenetic study of endophytic fungi associated with Centella asiatica from Bengkulu and Malaysian accessions based on the ITS rDNA sequence. Biodiversitas 20:  1248-1258. Centella asiatica is one of the medicinal plants which is known to be symbiotic with various endophytic fungi. The purpose of this research was to determine diversity of culturable fungal endophyte from C. asiatica. Identification was conducted using molecular phylogenetic analysis based on the ITS rDNA sequence. The result showed that obtained 145 isolates endophytic fungi (from stolons, leaves, roots, petioles) were grouped into 18 morphotaxa (Bengkulu) and 23 morphotaxa (Malaysian). The fungal endophytic were identified as Aspergillus austroafricanus, Aspergillus oryzae, Acrocalymma vagum, Ceratobasidium cornigerum, Ceratobasidium sp., Chaetomium globosum, Colletotrichum karstii, C. gigasporium, C. tabaci, Colletotrichum siamense, Eutypella sp., Fusarium solani, F. oxysporum, F. falciforme, F. keratoplasticum, F. striatum, Fusarium sp., Penicillium capsulatum, Phoma multirostrata, Perenniporia tephropora, Perenniporia sp., Phanerochaete chrysosporium, Phanerochaete stereoides, Phyllosticta capitalensis, Phomopsis asparagi, Peroneutypa scoparia, Phialemoniopsis sp., Mycochaetophora gentinae, Talaromyces sp., Earliella scabrosa, and Trichaptum sp. based on molecular phylogenetic analysis. Fusarium (Nectriaceae) were the most found of fungal endophytes in both C. asiatica Bengkulu and Malaysian accession. The majority strain are including Ascomycetes and Basidiomycetes. The identification of endophytic fungi from medicinal plants is needed as a preliminary study to determine the potential of endophytic fungi producing bioactive compounds.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...